[中国汉族老年成年人糖尿病与瘦素受体基因rs1137100和rs1137101多态性的关系]

[Relationship of diabetes mellitus in older Han adults in China with leptin receptor gene rs1137100 and rs1137101 polymorphrism].

作者信息

Wu Jinghuan, Zhuo Qin, Tian Yuan, Piao Jianhua, Yang Xiaoguang

机构信息

Key Laboratory of Trace Element Nutrition, National Health and Family Planning Commission, National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China.

出版信息

Wei Sheng Yan Jiu. 2017 May;46(3):384-388.

PMID:29903247

Abstract

OBJECTIVE

To explore the relationship of diabetes in older Han adults with leptin receptor gene rs1137100 and rs1137101 single nucleotide polymorphism( SNP).

METHODS

A total of 511 older Han adults were selected for this study and divided into diabetes and control groups, with 241 and 270 people in each group. The genotypes of rs1137100 and rs1137101 and the allele frequency were determined by Taqman method. The correlations between genotypes of leptin receptor gene SNPs with diabetes wereanalyzed. Blood concentrations of lipids, fasting serum glucose( FBG), leptin and insulin were also measured.

RESULTS

The distribution of GG, GA and AA genotypes in rs1137100 in the diabetes group and the healthy group was 73. 0%, 24. 5%, 2. 5% and 68. 9%, 28. 9%, 2. 2%, respectively. The difference was not significant between the two groups( χ~~2= 1. 27, P = 0. 53). There was no significance in the difference of the frequency of "A"allele in rs1137100 in the two groups, with 14. 7% and 16. 7% in each group( χ~~2= 0. 72, P = 0. 40). In rs1137101, the distribution of GG, GA and AA genotypes in the diabetes group and the healthy group was 77. 6%, 21. 2%, 1. 2% and 77. 8%, 21. 1%, 1. 1%, respectively. The difference was not significant between the two groups( χ~~2= 0. 02, P = 0. 99). There was no significance in the difference of the frequency of"A"allele in rs1137101 in the two groups, with 11. 8% 11. 7% in each group( χ~~2=0. 01, P = 0. 94. The Logistic analysis showed that GA and AA genotypes carriers in rs1137100 had similar risk for diabetes when comparing with the GG genotypes( OR =1. 06, 95% CI 0. 34-3. 34, OR = 0. 80, 95% CI 0. 54-1. 19). The GA and AA genotypes carriers in rs1137101 had similar risk for diabetes when comparing with the GG genotypes, and the OR values were 1. 12( 95% CI 0. 22-5. 63) and 1. 01( 95% CI0. 66-1. 54), respectively.

CONCLUSION

The rs1137100 and rs1137101 polymorphism are not significantly associated with the prevalence of diabetes in older Han adults.

摘要

目的

探讨汉族老年人群中瘦素受体基因rs1137100和rs1137101单核苷酸多态性（SNP）与糖尿病的关系。

方法

选取511例汉族老年人，分为糖尿病组和对照组，每组241例和270例。采用Taqman法检测rs1137100和rs1137101的基因型及等位基因频率。分析瘦素受体基因SNP基因型与糖尿病的相关性，并检测血脂、空腹血糖（FBG）、瘦素和胰岛素的血浓度。

结果

糖尿病组和健康组rs1137100的GG、GA和AA基因型分布分别为73.0%、24.5%、2.5%和68.9%、28.9%、2.2%，两组差异无统计学意义（χ² = 1.27，P = 0.53）。两组rs1137100中“A”等位基因频率差异无统计学意义，分别为14.7%和16.7%（χ² = 0.72，P = 0.40）。rs1137101中，糖尿病组和健康组的GG、GA和AA基因型分布分别为77.6%、21.2%、1.2%和77.8%、21.1%、1.1%，两组差异无统计学意义（χ² = 0.02，P = 0.99）。两组rs1137101中“A”等位基因频率差异无统计学意义，分别为11.8%和11.7%（χ² = 0.01，P = 0.94）。Logistic分析显示，rs1137100中GA和AA基因型携带者与GG基因型相比患糖尿病的风险相似（OR = 1.06，95%CI 0.34 - 3.34，OR = 0.80，95%CI 0.54 - 1.19）。rs1137101中GA和AA基因型携带者与GG基因型相比患糖尿病的风险相似，OR值分别为1.12（95%CI 0.22 - 5.63）和1.01（95%CI 0.66 - 1.54）。