番茄红素通过核因子红细胞 2 相关因子 2 信号通路减轻玉米赤霉烯酮诱导的仔猪睾丸支持细胞氧化损伤。
Lycopene attenuates zearalenone-induced oxidative damage of piglet sertoli cells through the nuclear factor erythroid-2 related factor 2 signaling pathway.
机构信息
College of Animal Science and Technology, Anhui Agricultural University, 130 West Changjiang Road, Hefei 230036, China.
College of Animal Science and Technology, Anhui Agricultural University, 130 West Changjiang Road, Hefei 230036, China; Key Laboratory of Animal Parasitology of Ministry of Agriculture, Laboratory of Quality and Safety Risk Assessment for Animal Products on Biohazards (Shanghai) of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
出版信息
Ecotoxicol Environ Saf. 2021 Dec 1;225:112737. doi: 10.1016/j.ecoenv.2021.112737. Epub 2021 Sep 3.
Zearalenone (ZEA) has an estrogenic effect and often causes reproductive damage. Pigs are particularly sensitive to it. Lycopene (LYC) is a type of fat-soluble natural carotenoid that has antioxidant, anti-inflammatory, anti-cancer, anti-cardiovascular and detoxifying effects. In this study, piglet sertoli cells (SCs) were used as research objects to investigate the mechanism of ZEA induced damage to piglet SCs and to evaluate the protective effect of LYC on ZEA induced toxic damage to piglet SCs. The results showed that ZEA damaged the cell structure and inhibited the expression of nuclear factor erythroid-2 related factor 2 (Nrf2) in the nucleus, which down-regulated the relative mRNA expression of heme oxygenase 1 (HO-1) and glutathione peroxidase 1 (GPX1) and decreased the activity of HO-1, glutathione peroxidase (GSH-Px) and total superoxide dismutase (T-SOD), resulting in an increase in malondialdehyde (MDA) and reactive oxygen species (ROS) content. ZEA downregulated the relative mRNA and protein expression of bcl-2 in piglet SCs, promoted cell apoptosis, and upregulated the relative mRNA and protein expression of LC3, beclin-1, and bax. After 3 h LYC-pretreatment, ZEA was added for mixed culture. The results of pretreatment with LYC showed that LYC could alleviate the cytotoxicity of ZEA to porlets SCs. Compared with ZEA group, improved the cell survival rate, promoted the expression of Nrf2 in the nucleus, upregulated the relative mRNA expression of HO-1 and GPX1, increased the activity of antioxidant enzymes, and reduced the levels of MDA and ROS. Moreover, after pretreatment with LYC, the mRNA expression of bcl-2 was upregulated, the apoptosis rate was decreased, the relative mRNA and protein expressions of LC3, beclin-1 and bax were downregulated, and autophagy was alleviated. In conclusion, LYC alleviated the oxidative damage of SCs caused by ZEA by promoting the expression of Nrf2 pathway and decreased autophagy and apoptosis.
玉米赤霉烯酮（ZEA）具有雌激素作用，常引起生殖损伤。猪对此尤为敏感。番茄红素（LYC）是一种脂溶性天然类胡萝卜素，具有抗氧化、抗炎、抗癌、抗心血管和解毒作用。本研究以仔猪睾丸支持细胞（SCs）为研究对象，探讨 ZEA 诱导仔猪 SCs 损伤的机制，评价 LYC 对 ZEA 诱导仔猪 SCs 毒性损伤的保护作用。结果表明，ZEA 损伤细胞结构，抑制核内核因子红细胞 2 相关因子 2（Nrf2）的表达，下调血红素加氧酶 1（HO-1）和谷胱甘肽过氧化物酶 1（GPX1）的相对 mRNA 表达，降低 HO-1、谷胱甘肽过氧化物酶（GSH-Px）和总超氧化物歧化酶（T-SOD）的活性，导致丙二醛（MDA）和活性氧（ROS）含量增加。ZEA 下调仔猪 SCs 中 bcl-2 的相对 mRNA 和蛋白表达，促进细胞凋亡，上调 LC3、beclin-1 和 bax 的相对 mRNA 和蛋白表达。LYC 预处理 3 h 后，加入 ZEA 进行混合培养。LYC 预处理结果表明，LYC 可减轻 ZEA 对仔猪 SCs 的细胞毒性。与 ZEA 组相比，提高了细胞存活率，促进了核内 Nrf2 的表达，上调了 HO-1 和 GPX1 的相对 mRNA 表达，增加了抗氧化酶的活性，降低了 MDA 和 ROS 的水平。此外，经 LYC 预处理后，bcl-2 的 mRNA 表达上调，凋亡率降低，LC3、beclin-1 和 bax 的相对 mRNA 和蛋白表达下调，自噬减轻。综上所述，LYC 通过促进 Nrf2 通路的表达，减轻 ZEA 引起的 SCs 氧化损伤，减少自噬和凋亡。
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