Lang Yifan, Li Kaihong, Meng Wei, Liu Renrong
School of Life Science, Jiangxi Science and Technology Normal University, Nanchang 330000, China.
Wei Sheng Yan Jiu. 2018 Mar;47(2):296-300.
To develop an indirect competitive chemiluminescent enzyme immunoassay( CLEIA) for the detection of bisphenol A in milk samples.
The CLEIA conditions including antigen coated concentration, concentration of methanol, concentration of enzyme labeled anti-antibody, p H and ionic strength were optimized to build competitive inhibition curve, determine the linear range and detection limit, and study the recovery of spiked milk samples.
In the standard curve of the optimized CLEIA, the half maximal inhibitory concentration( IC_(50)) was 3. 95 ng/m L and the limit of detection( LOD) was 0. 66 ng/m L. The CLEIA showed good recoveries with spiked milk ranging from 95. 0%-112. 9% with the relative standard deviation of0. 93%-3. 96%.
The proposed method has a high sensitivity and good specificity and is suitable for the determination of bisphenol A in milk samples.
建立一种间接竞争化学发光酶免疫分析法（CLEIA）用于检测牛奶样品中的双酚A。
优化CLEIA条件，包括抗原包被浓度、甲醇浓度、酶标抗抗体浓度、pH和离子强度，以建立竞争抑制曲线，确定线性范围和检测限，并研究加标牛奶样品的回收率。
在优化后的CLEIA标准曲线中，半数抑制浓度（IC₅₀）为3.95 ng/mL，检测限（LOD）为0.66 ng/mL。CLEIA对加标牛奶的回收率良好，范围为95.0% - 112.9%，相对标准偏差为0.93% - 3.96%。
所建立的方法具有高灵敏度和良好的特异性，适用于牛奶样品中双酚A的测定。
