College of Public Health, Zhengzhou University, No. 100 of Science Avenue, Zhengzhou 450001, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2011 Oct 15;81(1):544-7. doi: 10.1016/j.saa.2011.06.047. Epub 2011 Jul 6.
Sulfamethoxydiazine (SMD), which is often used for animal disease treatment, is harmful to human health. No SMD residue should be detected in food in some countries, such as USA and Japan. Therefore, it is significant to develop a high-throughput, high-sensitivity and accurate method for the determination of the content of SMD in food. In this paper, chemiluminescence enzyme immunoassay (CLEIA) was developed for quantification of SMD. For this method, the limit of detection was 3.2 pg/ml, the linear range was from 10 to 2000 pg/ml, the within-day and inter-day precision were below 13% and below 18%, respectively, and the recovery was from 85% to 105%. Milk and egg were selected as samples to be examined with this method, and the result indicated that this CLEIA method was suitable for screening and quality control of food.
磺胺二甲嘧啶（SMD）常用于动物疾病治疗，对人体健康有害。美国和日本等国家的食品中不应检测到 SMD 残留。因此，开发一种高通量、高灵敏度和准确的方法来测定食品中 SMD 的含量具有重要意义。本文建立了一种用于磺胺二甲嘧啶定量检测的化学发光酶免疫分析法（CLEIA）。该方法的检测限为 3.2pg/ml，线性范围为 10-2000pg/ml，日内和日间精密度均小于 13%，回收率为 85%-105%。选择牛奶和鸡蛋作为样品进行检验，结果表明该 CLEIA 方法适用于食品的筛选和质量控制。
