Institut de recherches cliniques de Montréal (affiliated to the Université de Montréal), 110 Avenue des Pins Ouest, Montréal, QC H2W 1R7, Canada.
Department of Nutrition, Faculty of Medicine, Université de Montréal, Montréal, Québec, Canada; Institut de recherches cliniques de Montréal (affiliated to the Université de Montréal), 110 Avenue des Pins Ouest, Montréal, QC H2W 1R7, Canada.
Cytokine. 2018 Nov;111:470-474. doi: 10.1016/j.cyto.2018.05.034. Epub 2018 Jun 11.
Chronic inflammation has been associated to the development of cardiometabolic dysfunctions. The use of an intravenous (IV) catheter is highly recommended for physiology testing. Yet, the presence of an IV catheter triggers local inflammation that does not reflect systemic inflammatory status. The aim of this study was to assess the effect of an IV catheter on serum concentrations of IL-6, IL-8 and hsCRP in a fasting state and after a high-fat meal known to trigger low-grade inflammation.
Twenty-two healthy subjects (7 men, 15 women) were included in this study. The trial included 2 visits. After an overnight fast, a venous catheter was inserted into an antecubital vein. A first blood sample was collected through this catheter at T = 0 min. On each visit, participants were requested either to drink only water for the whole duration of the test (WO test), or to consume a high-fat meal (HFM). Blood samples were collected through the catheter at T60, T120, T180 and T300 min. Additional venous punctures were performed on the contralateral arm at T180 and T300 min. Serum inflammatory mediators were measured at each time point of both interventions.
When serum was collected by venous punctures, IL-6 concentrations remained unchanged during both WO and HFM tests (P = 0.15 and P = 0.23, respectively), whereas the concentrations increased progressively over time when serum was collected through the catheter (P < 0.001). The high-fat meal had no additional effect on IL-6 levels (P = 0.27) neither in serum collected by venous puncture nor in serum collected through the catheter. Serum IL-8 and hsCRP concentrations did not vary over time, and were influenced neither by the meal type nor by the blood collection method.
The insertion of an indwelling catheter is associated with a local inflammatory response possibly mediated by IL-6 but not IL-8. This inflammatory response was not enhanced by a pro-inflammatory high-fat meal.
慢性炎症与心脏代谢功能障碍的发展有关。静脉（IV）导管的使用强烈建议用于生理学测试。然而，IV 导管的存在会引发局部炎症，而不会反映全身炎症状态。本研究旨在评估在空腹状态下和已知会引发低度炎症的高脂肪餐后，IV 导管对血清中白细胞介素 6（IL-6）、白细胞介素 8（IL-8）和高敏 C 反应蛋白（hsCRP）浓度的影响。
本研究纳入了 22 名健康受试者（7 名男性，15 名女性）。试验包括 2 次就诊。受试者在过夜禁食后，在前臂静脉中插入静脉导管。在 T=0 分钟时，通过该导管采集第一份血样。在每次就诊时，要求参与者要么在整个测试期间只饮水（WO 测试），要么摄入高脂肪餐（HFM）。在 T60、T120、T180 和 T300 分钟时，通过导管采集血样。在 T180 和 T300 分钟时，在对侧手臂进行额外的静脉穿刺。在两种干预措施的每个时间点测量血清炎症介质。
当通过静脉穿刺采集血清时，IL-6 浓度在 WO 和 HFM 试验中均保持不变（P=0.15 和 P=0.23），而当通过导管采集血清时，浓度随着时间的推移而逐渐增加（P<0.001）。高脂肪餐对静脉穿刺采集的血清中 IL-6 水平（P=0.27）或导管采集的血清中 IL-6 水平均无额外影响。血清 IL-8 和 hsCRP 浓度随时间变化而变化，不受餐型或采血方法的影响。
留置导管与局部炎症反应有关，这种炎症反应可能由白细胞介素 6 介导，但不是白细胞介素 8 介导。这种炎症反应不受促炎高脂肪餐的增强。
