Trudinger P A, Meyer T E, Bartsch R G, Kamen M D
Arch Microbiol. 1985 May;141(4):273-8. doi: 10.1007/BF00428836.
Four cytochromes were isolated from soluble extracts of the aerobic sulfur bacterium, Thiobacillus neapolitanus. The two most abundant proteins were purified to homogeneity and thoroughly characterized. Cytochrome c-554 (547) is a monomeric, small molecular weight protein which is unusual in having two well-resolved alpha peaks in UV-visible absorption spectra. The redox potential is 208 mV. Native cytochrome c-549 is oligomeric, but has a subunit size of about 26,000. The yield of this protein could be improved dramatically by washing membranes with 30% ammonium sulfate, but the material solubilized by this method had a larger native molecular weight than that in the initial 0.1 M Tris-Cl extract and behaved differently on chromatography. The properties of cytochrome c-549 including subunit size and UV-visible absorption spectra are similar to mitochondrial cytochrome c1 and chloroplast cytochrome f, which suggests that it may be a modified form of the predominant membrane cytochrome. Based on cytochrome content, it is suggested that T. neapolitanus is not closely related to other thiobacilli.
从嗜氧硫细菌那不勒斯硫杆菌的可溶性提取物中分离出了四种细胞色素。两种含量最丰富的蛋白质被纯化至同质，并进行了全面表征。细胞色素c-554（547）是一种单体小分子蛋白质，其不同寻常之处在于在紫外可见吸收光谱中有两个分辨率良好的α峰。氧化还原电位为208毫伏。天然细胞色素c-549是寡聚体，但亚基大小约为26000。用30%硫酸铵洗涤膜可显著提高该蛋白质的产量，但用这种方法溶解的物质的天然分子量比初始0.1M Tris-Cl提取物中的大，并且在色谱上表现不同。细胞色素c-549的性质，包括亚基大小和紫外可见吸收光谱，与线粒体细胞色素c1和叶绿体细胞色素f相似，这表明它可能是主要膜细胞色素的一种修饰形式。基于细胞色素含量，有人认为那不勒斯硫杆菌与其他硫杆菌关系不密切。
