Kaguni J M, Bertsch L L, Bramhill D, Flynn J E, Fuller R S, Funnell B, Maki S, Ogawa T, Ogawa K, van der Ende A
Basic Life Sci. 1985;30:141-50. doi: 10.1007/978-1-4613-2447-8_14.
A mixture of purified proteins has replaced a crude enzyme fraction capable of efficient replication of oriC-containing plasmids. The reconstituted enzyme system contains proteins which provide initiation, replication, and specificity functions required for dnaA-dependent replication specific for an oriC template. Replication can be separated into successive stages of RNA synthesis and DNA replication. Isolation of an intermediate no longer requiring RNA polymerase action requires the presence of dnaA protein, DNA gyrase, dnaB protein and dnaC protein. Intermediate formation likely involves binding of dnaA protein to a 9-bp sequence present 4 times as inverted repeats within the chromosomal origin sequence.
一种纯化蛋白质的混合物已经取代了能够高效复制含oriC质粒的粗酶组分。重构的酶系统包含一些蛋白质，这些蛋白质提供了oriC模板特异性的、依赖于dnaA的复制所需的起始、复制和特异性功能。复制可以分为RNA合成和DNA复制的连续阶段。分离不再需要RNA聚合酶作用的中间体需要dnaA蛋白、DNA旋转酶、dnaB蛋白和dnaC蛋白的存在。中间体的形成可能涉及dnaA蛋白与染色体起源序列中以反向重复形式出现4次的9碱基对序列的结合。
