Funnell B E, Baker T A, Kornberg A
J Biol Chem. 1987 Jul 25;262(21):10327-34.
During initiation of DNA replication of plasmids containing the origin of the Escherichia coli chromosome (oriC), the proteins dnaA, dnaB, and dnaC interact and assemble a complex at oriC. The complex is larger and more asymmetric than that formed by dnaA protein and embraces an extra 50 base pairs at the left side of the minimal oriC sequence. Both dnaA and dnaB proteins have been identified in the complex by electron microscopy and antibody binding; dnaC protein was not detected. HU protein, which stimulates the activity of the initiation reaction, was often present. Entry of dnaB protein required dnaA and dnaC proteins and a supercoiled template. Thus, a complex structure, involving multiple proteins and a large region of DNA, must be formed at the origin to prepare the template for priming and replication.
在含有大肠杆菌染色体复制起点（oriC）的质粒进行DNA复制起始过程中，蛋白质DnaA、DnaB和DnaC相互作用并在oriC处组装成一个复合物。该复合物比由DnaA蛋白形成的复合物更大且更不对称，并且在最小oriC序列左侧包含额外的50个碱基对。通过电子显微镜和抗体结合在复合物中鉴定出了DnaA和DnaB蛋白；未检测到DnaC蛋白。经常存在刺激起始反应活性的HU蛋白。DnaB蛋白的进入需要DnaA和DnaC蛋白以及一个超螺旋模板。因此，必须在复制起点形成一个涉及多种蛋白质和大片段DNA的复杂结构，以为引发和复制准备模板。
