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实验室课程中人类口腔微生物群样本分子指纹识别的简易方案。

Simple Protocol for Molecular Fingerprinting of Human Oral Microbiota Samples in Lab Classes.

作者信息

Henriques Ana C, De Marco Paolo

机构信息

CESPU, Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde (IINFACTS), 4585-116 Gandra PRD, Portugal.

出版信息

J Microbiol Biol Educ. 2018 Jan 26;19(1). doi: 10.1128/jmbe.v19i1.1305. eCollection 2018.

DOI:10.1128/jmbe.v19i1.1305
PMID:29904507
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5969393/
Abstract

DNA fingerprinting is a major tool in identifying individuals and in evidence matching. However, this technique can be difficult to reproduce in practical classes. Here, we report on distinct PCR profiles obtained when amplifying saliva DNA of a score of distinct individuals with Random Amplified Polymorphic DNA (RAPD)-PCR primer BOXA1R. The RAPD-PCR method is simple and efficient for discrimination between bacterial strains and is used in this instance to obtain personalized fingerprints of each individual's oral microbiota. We present real results with undergraduate students confirming that this procedure is easily feasible in practical classes. Based on the results presented, we suggest a laboratory activity for undergraduate Molecular Biology or Microbiology students.

摘要

DNA指纹识别是识别个体和进行证据匹配的主要工具。然而，这项技术在实践课程中可能难以重现。在此，我们报告了使用随机扩增多态性DNA（RAPD）-PCR引物BOXA1R扩增多个不同个体的唾液DNA时获得的独特PCR图谱。RAPD-PCR方法对于区分细菌菌株简单有效，在此用于获得每个人口腔微生物群的个性化指纹。我们展示了与本科生的实际结果，证实了该程序在实践课程中很容易实现。基于所呈现的结果，我们为本科分子生物学或微生物学学生建议了一项实验室活动。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9df5/5969393/94ef2cd6fa37/jmbe-19-1f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9df5/5969393/ff33bd915386/jmbe-19-1f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9df5/5969393/94ef2cd6fa37/jmbe-19-1f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9df5/5969393/ff33bd915386/jmbe-19-1f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9df5/5969393/94ef2cd6fa37/jmbe-19-1f2.jpg

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本文引用的文献

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