H3K27ac建立过程中的DNA序列特征

DNA sequence features in the establishing of H3K27ac.

作者信息

Zubritskiy Anatoliy, Medvedeva Yulia A

机构信息

Institute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences, Moscow, 119071, Russian Federation.

Department of Computational Biology, Vavilov institute of General Genetics, Russian Academy of Sciences, Moscow, 119991, Russian Federation.

出版信息

F1000Res. 2018 Feb 8;7:165. doi: 10.12688/f1000research.13441.2. eCollection 2018.

DOI:10.12688/f1000research.13441.2

PMID:29904589

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5989146/

Abstract

The presence of H3K27me3 has been demonstrated to correlate with the CpG content. In this work, we tested whether H3K27ac has similar sequence preferences. We performed a translocation of DNA sequences with various properties into a beta-globin locus to control for the local chromatin environment. Our results suggest that in contrast to H3K27me3, H3K27ac gain is unlikely affected by the CpG content of the underlying DNA sequence, while extremely high GC-content might contribute to the gain of the H3K27ac.

摘要

已有研究表明H3K27me3的存在与CpG含量相关。在本研究中，我们测试了H3K27ac是否具有类似的序列偏好性。我们将具有各种特性的DNA序列易位到β-珠蛋白基因座，以控制局部染色质环境。我们的结果表明，与H3K27me3不同，H3K27ac的增加不太可能受潜在DNA序列的CpG含量影响，而极高的GC含量可能有助于H3K27ac的增加。