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使用颗粒掺杂组织样本对一种新型颗粒分离程序进行验证。

Validation of a novel particle isolation procedure using particle doped tissue samples.

作者信息

Patel J, Lal S, Wilshaw S P, Hall R M, Tipper J L

机构信息

Faculty of Biological Sciences, University of Leeds, UK.

School of Mechanical Engineering, University of Leeds, UK.

出版信息

Data Brief. 2018 May 1;18:1802-1807. doi: 10.1016/j.dib.2018.04.096. eCollection 2018 Jun.

DOI:10.1016/j.dib.2018.04.096
PMID:29904681
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5998301/
Abstract

A novel particle isolation method for tissue samples was developed and tested using particle-doped peri-articular tissues from ovine cadavers. This enabled sensitivity of the isolation technique to be established by doping tissue samples of 0.25 g with very low particle volumes of 2.5 µm per sample. Image analysis was used to verify that the method caused no changes to particle size or morphologies.

摘要

开发了一种用于组织样本的新型颗粒分离方法,并使用来自绵羊尸体的颗粒掺杂关节周围组织进行了测试。通过向0.25克组织样本中掺杂每个样本极低体积的2.5微米颗粒,能够确定分离技术的灵敏度。使用图像分析来验证该方法不会改变颗粒大小或形态。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3389/5998301/28f0488d0cf9/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3389/5998301/1779ae5504cd/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3389/5998301/584e8ca7e6b0/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3389/5998301/28f0488d0cf9/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3389/5998301/1779ae5504cd/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3389/5998301/584e8ca7e6b0/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3389/5998301/28f0488d0cf9/gr3.jpg

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本文引用的文献

1
Recovery of low volumes of wear debris from rat stifle joint tissues using a novel particle isolation method.使用新型颗粒分离方法从大鼠膝关节组织中回收少量磨损颗粒。
Acta Biomater. 2018 Apr 15;71:339-350. doi: 10.1016/j.actbio.2018.02.030. Epub 2018 Mar 2.
2
Comparison of the cytotoxicity of clinically relevant cobalt-chromium and alumina ceramic wear particles in vitro.临床相关钴铬合金与氧化铝陶瓷磨损颗粒体外细胞毒性的比较。
Biomaterials. 2003 Feb;24(3):469-79. doi: 10.1016/s0142-9612(02)00360-5.