可调角蛋白水凝胶侵蚀对重组人骨形态发生蛋白 2 释放、生物活性和骨诱导的影响。
Effects of Tunable Keratin Hydrogel Erosion on Recombinant Human Bone Morphogenetic Protein 2 Release, Bioactivity, and Bone Induction.
机构信息
1 Department of Biomedical Engineering, Virginia Commonwealth University , Richmond, Virginia.
2 Department of Chemical, Paper and Biomedical Engineering, College of Engineering and Computing, Miami University , Oxford, Ohio.
出版信息
Tissue Eng Part A. 2018 Nov;24(21-22):1616-1630. doi: 10.1089/ten.TEA.2017.0471. Epub 2018 Sep 6.
Recombinant human bone morphogenetic protein 2 (rhBMP-2) delivery from collagen sponges for bone formation is an important clinical example of growth factors in tissue engineering. Side effects from rhBMP-2 burst release and rapid collagen resorption have led to investigation of alternative carriers. Here, keratin carriers with tunable erosion rates were formulated by varying disulfide crosslinking via ratios of oxidatively (keratose) to reductively (kerateine) extracted keratin. In vitro rhBMP-2 bioactivity increased with kerateine content, reaching levels greater than with collagen. Heterotopic bone formation in a mouse model depended on the keratin formulation, highlighting the importance of the growth factor carrier.
胶原海绵中重组人骨形态发生蛋白 2（rhBMP-2）的释放对于骨形成是组织工程中生长因子的一个重要临床范例。rhBMP-2 爆发释放和胶原快速吸收的副作用导致了对替代载体的研究。在这里，通过氧化（角蛋白）和还原（角蛋白氨酸）提取角蛋白的比例来改变二硫键交联，从而制备了具有可调节侵蚀率的角蛋白载体。体外 rhBMP-2 生物活性随角蛋白氨酸含量的增加而增加，达到高于胶原的水平。在小鼠模型中的异位骨形成取决于角蛋白配方，这突出了生长因子载体的重要性。
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