可调角蛋白水凝胶侵蚀对重组人骨形态发生蛋白 2 释放、生物活性和骨诱导的影响。
Effects of Tunable Keratin Hydrogel Erosion on Recombinant Human Bone Morphogenetic Protein 2 Release, Bioactivity, and Bone Induction.
机构信息
1 Department of Biomedical Engineering, Virginia Commonwealth University , Richmond, Virginia.
2 Department of Chemical, Paper and Biomedical Engineering, College of Engineering and Computing, Miami University , Oxford, Ohio.
出版信息
Tissue Eng Part A. 2018 Nov;24(21-22):1616-1630. doi: 10.1089/ten.TEA.2017.0471. Epub 2018 Sep 6.
Abstract
Recombinant human bone morphogenetic protein 2 (rhBMP-2) delivery from collagen sponges for bone formation is an important clinical example of growth factors in tissue engineering. Side effects from rhBMP-2 burst release and rapid collagen resorption have led to investigation of alternative carriers. Here, keratin carriers with tunable erosion rates were formulated by varying disulfide crosslinking via ratios of oxidatively (keratose) to reductively (kerateine) extracted keratin. In vitro rhBMP-2 bioactivity increased with kerateine content, reaching levels greater than with collagen. Heterotopic bone formation in a mouse model depended on the keratin formulation, highlighting the importance of the growth factor carrier.
摘要
胶原海绵中重组人骨形态发生蛋白 2（rhBMP-2）的释放对于骨形成是组织工程中生长因子的一个重要临床范例。rhBMP-2 爆发释放和胶原快速吸收的副作用导致了对替代载体的研究。在这里，通过氧化（角蛋白）和还原（角蛋白氨酸）提取角蛋白的比例来改变二硫键交联，从而制备了具有可调节侵蚀率的角蛋白载体。体外 rhBMP-2 生物活性随角蛋白氨酸含量的增加而增加，达到高于胶原的水平。在小鼠模型中的异位骨形成取决于角蛋白配方，这突出了生长因子载体的重要性。
相似文献
1
Effects of Tunable Keratin Hydrogel Erosion on Recombinant Human Bone Morphogenetic Protein 2 Release, Bioactivity, and Bone Induction.可调角蛋白水凝胶侵蚀对重组人骨形态发生蛋白 2 释放、生物活性和骨诱导的影响。
Tissue Eng Part A. 2018 Nov;24(21-22):1616-1630. doi: 10.1089/ten.TEA.2017.0471. Epub 2018 Sep 6.
2
Alkylation of human hair keratin for tunable hydrogel erosion and drug delivery in tissue engineering applications.用于组织工程应用中可调节水凝胶侵蚀和药物递送的人发角蛋白烷基化
Acta Biomater. 2015 Sep;23:201-213. doi: 10.1016/j.actbio.2015.05.013. Epub 2015 May 18.
3
Tunable Keratin Hydrogels for Controlled Erosion and Growth Factor Delivery.用于可控侵蚀和生长因子递送的可调节角蛋白水凝胶
Biomacromolecules. 2016 Jan 11;17(1):225-36. doi: 10.1021/acs.biomac.5b01328. Epub 2015 Dec 14.
4
Reduction of ectopic bone growth in critically-sized rat mandible defects by delivery of rhBMP-2 from kerateine biomaterials.通过角蛋白生物材料递送重组人骨形态发生蛋白-2减少大鼠临界大小下颌骨缺损中的异位骨生长
Biomaterials. 2014 Mar;35(10):3220-8. doi: 10.1016/j.biomaterials.2013.12.087. Epub 2014 Jan 15.
5
Programmed Sustained Release of Recombinant Human Bone Morphogenetic Protein-2 and Inorganic Ion Composite Hydrogel as Artificial Periosteum.作为人工骨膜的重组人骨形态发生蛋白-2和无机离子复合水凝胶的程序性持续释放。
ACS Appl Mater Interfaces. 2020 Feb 12;12(6):6840-6851. doi: 10.1021/acsami.9b18496. Epub 2020 Jan 30.
6
Keratin hydrogel carrier system for simultaneous delivery of exogenous growth factors and muscle progenitor cells.用于同时递送外源性生长因子和肌肉祖细胞的角蛋白水凝胶载体系统。
J Biomed Mater Res B Appl Biomater. 2016 Jul;104(5):864-79. doi: 10.1002/jbm.b.33438. Epub 2015 May 7.
7
Evaluation of a Novel HA/ZrO2-Based Porous Bioceramic Artificial Vertebral Body Combined with a rhBMP-2/Chitosan Slow-Release Hydrogel.一种新型HA/ZrO₂基多孔生物陶瓷人工椎体与rhBMP-2/壳聚糖缓释水凝胶联合应用的评估
PLoS One. 2016 Jul 11;11(7):e0157698. doi: 10.1371/journal.pone.0157698. eCollection 2016.
8
Design of hydrogels to stabilize and enhance bone morphogenetic protein activity by heparin mimetics.通过肝素类似物设计水凝胶以稳定和增强骨形态发生蛋白活性。
Acta Biomater. 2018 May;72:45-54. doi: 10.1016/j.actbio.2018.03.034. Epub 2018 Mar 26.
9
Ectopic osteogenesis effect of antigen-extracted xenogeneic cancellous bone graft with chitosan/rhBMP-2/bFGF sequential sustained-release nanocapsules.壳聚糖/重组人骨形态发生蛋白-2/碱性成纤维细胞生长因子序贯缓释纳米囊抗原提取异种松质骨移植的异位成骨效应
J Biomater Appl. 2018 Jul;33(1):23-43. doi: 10.1177/0885328218761193. Epub 2018 Mar 2.
10
Sustained release of rhBMP-2 from microporous tricalciumphosphate using hydrogels as a carrier.使用水凝胶作为载体从微孔磷酸三钙中持续释放重组人骨形态发生蛋白-2。
BMC Biotechnol. 2016 May 20;16(1):44. doi: 10.1186/s12896-016-0275-8.
引用本文的文献
1
Rapidly Polymerizing Click Hydrogel Provides Localized Delivery of rhBMP2 to Promote Bone Formation.快速聚合点击水凝胶实现rhBMP2的局部递送以促进骨形成。
Pharmacol Res Perspect. 2025 Jun;13(3):e70119. doi: 10.1002/prp2.70119.
2
Sustainable Applications of Animal Waste Proteins.动物粪便蛋白质的可持续应用。
Polymers (Basel). 2022 Apr 14;14(8):1601. doi: 10.3390/polym14081601.
3
Design and Characterization of Biomimetic Kerateine Aerogel-Electrospun Polycaprolactone Scaffolds for Retinal Cell Culture.仿生角蛋白气凝胶-静电纺聚己内酯支架的设计与表征及其用于视网膜细胞培养。
Ann Biomed Eng. 2021 Jul;49(7):1633-1644. doi: 10.1007/s10439-021-02756-5. Epub 2021 Apr 6.
4
Natural Polymeric Scaffolds in Bone Regeneration.用于骨再生的天然高分子支架
Front Bioeng Biotechnol. 2020 May 21;8:474. doi: 10.3389/fbioe.2020.00474. eCollection 2020.
5
New Developments in Medical Applications of Hybrid Hydrogels Containing Natural Polymers.新型天然高分子杂化水凝胶在医学中的应用进展。
Molecules. 2020 Mar 27;25(7):1539. doi: 10.3390/molecules25071539.
本文引用的文献
1
Keratin Hydrogel Enhances In Vivo Skeletal Muscle Function in a Rat Model of Volumetric Muscle Loss.角蛋白水凝胶增强体积性肌肉损失大鼠模型的体内骨骼肌功能。
Tissue Eng Part A. 2017 Jun;23(11-12):556-571. doi: 10.1089/ten.TEA.2016.0458. Epub 2017 Apr 14.
2
Cell and Growth Factor-Loaded Keratin Hydrogels for Treatment of Volumetric Muscle Loss in a Mouse Model.用于治疗小鼠模型中体积性肌肉损失的细胞和生长因子负载角蛋白水凝胶
Tissue Eng Part A. 2017 Jun;23(11-12):572-584. doi: 10.1089/ten.TEA.2016.0457. Epub 2017 Apr 14.
3
Delivery vehicle effects on bone regeneration and heterotopic ossification induced by high dose BMP-2.递送载体对高剂量骨形态发生蛋白-2诱导的骨再生和异位骨化的影响。
Acta Biomater. 2017 Feb;49:101-112. doi: 10.1016/j.actbio.2016.12.012. Epub 2016 Dec 8.
4
Change in Off-Label Use of Bone Morphogenetic Protein in Spine Surgery and Associations with Adverse Outcome.脊柱手术中骨形态发生蛋白的非标签使用变化及其与不良结局的关联。
Global Spine J. 2016 Nov;6(7):650-659. doi: 10.1055/s-0036-1571284. Epub 2016 Jan 15.
5
Tunable Keratin Hydrogels for Controlled Erosion and Growth Factor Delivery.用于可控侵蚀和生长因子递送的可调节角蛋白水凝胶
Biomacromolecules. 2016 Jan 11;17(1):225-36. doi: 10.1021/acs.biomac.5b01328. Epub 2015 Dec 14.
6
Regenerative medicine: Current therapies and future directions.再生医学：当前疗法与未来方向。
Proc Natl Acad Sci U S A. 2015 Nov 24;112(47):14452-9. doi: 10.1073/pnas.1508520112.
7
Binding Interactions of Keratin-Based Hair Fiber Extract to Gold, Keratin, and BMP-2.基于角蛋白的头发纤维提取物与金、角蛋白和骨形态发生蛋白-2的结合相互作用。
PLoS One. 2015 Aug 28;10(8):e0137233. doi: 10.1371/journal.pone.0137233. eCollection 2015.
8
Alkylation of human hair keratin for tunable hydrogel erosion and drug delivery in tissue engineering applications.用于组织工程应用中可调节水凝胶侵蚀和药物递送的人发角蛋白烷基化
Acta Biomater. 2015 Sep;23:201-213. doi: 10.1016/j.actbio.2015.05.013. Epub 2015 May 18.
9
Keratin hydrogel carrier system for simultaneous delivery of exogenous growth factors and muscle progenitor cells.用于同时递送外源性生长因子和肌肉祖细胞的角蛋白水凝胶载体系统。
J Biomed Mater Res B Appl Biomater. 2016 Jul;104(5):864-79. doi: 10.1002/jbm.b.33438. Epub 2015 May 7.
10
Preparation of keratin and chemically modified keratin hydrogels and their evaluation as cell substrate with drug releasing ability.角蛋白及化学修饰角蛋白水凝胶的制备及其作为具有药物释放能力的细胞基质的评价。
J Biosci Bioeng. 2015 Jul;120(1):111-6. doi: 10.1016/j.jbiosc.2014.12.005. Epub 2015 Jan 3.
Zotero 插件