Nakudashvili Z, Mgebrishvili I, Barbakadze I, Sanikidze T
Tbilisi State Medical University; I. Javakhishvili Tbilisi State University, Georgia.
Georgian Med News. 2018 May(278):50-55.
The purpose of our study was a comparative analysis of the effect of dentures from various materials on the immunological and redox-dependent homeostasis of the oral cavity. We studied 60 patients with removable dentures made based on plastics Prothyl Hot, Vertex BasiQ 20 (differing by polymerization regime) and elastic thermoplastic polymer Perflex Flexi Nylon. The control group consisted of 15 volunteers with a practically healthy oral cavity, who did not use dentures. Saliva collected on an empty stomach in a glass tube without the use of a stimulator before the establishment of a denture and 3 days and 1 month after. The content of the protein P-53 in saliva determined by immunoenzymatic assay with use of "Cusabio" reagent. The cytokines (IL1β, IL10) content in saliva was determined immunoenzymatic assay. To determine the redox balance in the saliva of patients, the lipoperoxydradicals content (LOO.) content (by EPR method, using the spin-labeled α-phenyl-tertbutylnitron (PBN) (SIGMA)) and the activity of antioxidant enzymes (catalase and SOD) (by spectrophotometry) studied. Statistical processing of the results was carried out using the software package SPSS (version 10.0). Results of analysis show that defects associated with a lack of teeth do not affect the immune and oxidative balance of the oral cavity, but contribute to the development of destructive changes in the oral cavity's soft tissues, which manifested by an increase in the content of the proapoptotic protein P-53 in the saliva. After establishment of a denture, the intensity of apoptosis in the oral cavity tissues reduced. Establishment of a denture induced development of an inflammatory reaction during the first days, the intensity of which gradually decreased and completely disappeared at the end of the first month of the observation (manifested by the normalization of the parameters of the immune balance and antioxidant system). Minimal traumatic effects observed during establishment of a denture made based on Perflex Flexi Nylon.
我们研究的目的是对不同材料制成的假牙对口腔免疫和氧化还原依赖性内环境稳定的影响进行比较分析。我们研究了60例佩戴基于Prothyl Hot塑料、Vertex BasiQ 20（因聚合方式不同）和弹性热塑性聚合物Perflex Flexi Nylon制成的可摘假牙的患者。对照组由15名口腔基本健康且未佩戴假牙的志愿者组成。在佩戴假牙前、佩戴后3天和1个月，于空腹状态下在不使用刺激剂的情况下，用玻璃管收集唾液。使用“Cusabio”试剂通过免疫酶测定法测定唾液中蛋白质P - 53的含量。通过免疫酶测定法测定唾液中细胞因子（IL1β、IL10）的含量。为了确定患者唾液中的氧化还原平衡，研究了脂过氧化自由基含量（LOO.）（通过电子顺磁共振方法，使用自旋标记的α - 苯基 - 叔丁基硝基（PBN）（西格玛））和抗氧化酶（过氧化氢酶和超氧化物歧化酶）的活性（通过分光光度法）。使用软件包SPSS（版本10.0）对结果进行统计处理。分析结果表明，与牙齿缺失相关的缺陷不会影响口腔的免疫和氧化平衡，但会导致口腔软组织发生破坏性变化，表现为唾液中促凋亡蛋白P - 53含量增加。佩戴假牙后，口腔组织中的凋亡强度降低。佩戴假牙在最初几天会引发炎症反应，其强度逐渐降低，并在观察的第一个月末完全消失（表现为免疫平衡和抗氧化系统参数恢复正常）。基于Perflex Flexi Nylon制成的假牙在佩戴过程中观察到的创伤影响最小。
