宽场压缩多光子显微镜。
Widefield compressive multiphoton microscopy.
出版信息
Opt Lett. 2018 Jun 15;43(12):2989-2992. doi: 10.1364/OL.43.002989.
Abstract
A single-pixel compressively sensed architecture is exploited to simultaneously achieve a 10× reduction in acquired data compared with the Nyquist rate, while alleviating limitations faced by conventional widefield temporal focusing microscopes due to scattering of the fluorescence signal. Additionally, we demonstrate an adaptive sampling scheme that further improves the compression and speed of our approach.
摘要
利用单像素压缩感知架构，与奈奎斯特速率相比，同时实现采集数据减少 10 倍，同时缓解由于荧光信号散射而导致传统宽场时聚焦显微镜面临的限制。此外，我们还展示了一种自适应采样方案，进一步提高了我们方法的压缩和速度。
相似文献
1
Widefield compressive multiphoton microscopy.宽场压缩多光子显微镜。
Opt Lett. 2018 Jun 15;43(12):2989-2992. doi: 10.1364/OL.43.002989.
3
Optimal compressive multiphoton imaging at depth using single-pixel detection.利用单像素探测实现深层最优压缩多光子成像。
Opt Lett. 2019 Oct 15;44(20):4981-4984. doi: 10.1364/OL.44.004981.
4
Ultrafast widefield optical sectioning microscopy by multifocal temporal focusing.基于多焦点时间聚焦的超快宽视场光学切片显微镜术
Opt Express. 2010 Sep 13;18(19):19645-55. doi: 10.1364/OE.18.019645.
5
Temporal focusing-based widefield multiphoton microscopy with spatially modulated illumination for biotissue imaging.基于时间聚焦并采用空间调制照明的宽场多光子显微镜用于生物组织成像。
J Biophotonics. 2018 Jan;11(1). doi: 10.1002/jbio.201600287. Epub 2017 May 2.
6
Widefield multiphoton and temporally decorrelated multifocal multiphoton microscopy.宽场多光子和时间去相关多焦点多光子显微镜术
Opt Express. 2000 Oct 9;7(8):273-9. doi: 10.1364/oe.7.000273.
7
Super Sub-Nyquist Single-Pixel Imaging by Total Variation Ascending Ordering of the Hadamard Basis.基于哈达玛基序的全变差升序排列实现超奈奎斯特单像素成像
Sci Rep. 2020 Jun 9;10(1):9338. doi: 10.1038/s41598-020-66371-5.
8
Beating Nyquist with light: a compressively sampled photonic link.用光突破奈奎斯特极限：一种压缩采样光子链路。
Opt Express. 2011 Apr 11;19(8):7339-48. doi: 10.1364/OE.19.007339.
9
Temporal focusing multiphoton microscopy with optimized parallel multiline scanning for fast biotissue imaging.优化并行多线扫描的时聚焦多光子显微镜用于快速生物组织成像。
J Biomed Opt. 2021 Jan;26(1). doi: 10.1117/1.JBO.26.1.016501.
10
Two-dimensional spatiotemporal focusing of femtosecond pulses and its applications in microscopy.飞秒脉冲的二维时空聚焦及其在显微镜学中的应用。
Rev Sci Instrum. 2015 Aug;86(8):083701. doi: 10.1063/1.4927532.
引用本文的文献
1
Remote Focusing in a Temporal Focusing Microscope.时间聚焦显微镜中的远程聚焦
OSA Contin. 2021 Nov 15;4(11):2757-2770. doi: 10.1364/osac.443116. Epub 2021 Oct 27.
2
Deep Compressed Imaging via Optimized-Pattern Scanning.通过优化模式扫描实现深度压缩成像
Photonics Res. 2021 Mar 1;9(3):B57-B70. doi: 10.1364/prj.410556. Epub 2021 Jan 15.
3
De-scattering with Excitation Patterning enables rapid wide-field imaging through scattering media.采用激发图案化的去散射技术可实现通过散射介质的快速宽视场成像。
Sci Adv. 2021 Jul 7;7(28). doi: 10.1126/sciadv.aay5496. Print 2021 Jul.
4
Single-pixel imaging of dynamic objects using multi-frame motion estimation.使用多帧运动估计进行动态物体的单像素成像。
Sci Rep. 2021 Apr 8;11(1):7712. doi: 10.1038/s41598-021-83810-z.
5
Demonstration of flat-top beam illumination in widefield multiphoton microscopy.演示宽场多光子显微镜中的平顶光束照明。
J Biomed Opt. 2019 Nov;25(1):1-8. doi: 10.1117/1.JBO.25.1.014503.
本文引用的文献
1
Wide-field multiphoton imaging through scattering media without correction.未校正情况下通过散射介质的宽场多光子成像。
Sci Adv. 2018 Oct 12;4(10):eaau1338. doi: 10.1126/sciadv.aau1338. eCollection 2018 Oct.
2
Temporally precise single-cell-resolution optogenetics.时间精确的单细胞分辨率光遗传学
Nat Neurosci. 2017 Dec;20(12):1796-1806. doi: 10.1038/s41593-017-0018-8. Epub 2017 Nov 13.
3
Adaptive foveated single-pixel imaging with dynamic supersampling.自适应注视点单像素成像与动态超采样。
Sci Adv. 2017 Apr 21;3(4):e1601782. doi: 10.1126/sciadv.1601782. eCollection 2017 Apr.
4
Wide field-of-view, multi-region, two-photon imaging of neuronal activity in the mammalian brain.哺乳动物大脑神经元活动的宽视野、多区域双光子成像。
Nat Biotechnol. 2016 Aug;34(8):857-62. doi: 10.1038/nbt.3594. Epub 2016 Jun 27.
5
Ultrafast axial scanning for two-photon microscopy via a digital micromirror device and binary holography.通过数字微镜器件和二元全息术实现用于双光子显微镜的超快轴向扫描。
Opt Lett. 2016 Apr 1;41(7):1451-4. doi: 10.1364/OL.41.001451.
6
Fast 3-D temporal focusing microscopy using an electrically tunable lens.使用电可调透镜的快速三维时间聚焦显微镜。
Opt Express. 2015 Sep 21;23(19):24362-8. doi: 10.1364/OE.23.024362.
7
High-speed flow microscopy using compressed sensing with ultrafast laser pulses.使用压缩感知与超快激光脉冲的高速流动显微镜术。
Opt Express. 2015 Apr 20;23(8):10521-32. doi: 10.1364/OE.23.010521.
8
Nonlinear structured-illumination enhanced temporal focusing multiphoton excitation microscopy with a digital micromirror device.基于数字微镜器件的非线性结构光照增强时间聚焦多光子激发显微镜
Biomed Opt Express. 2014 Jul 8;5(8):2526-36. doi: 10.1364/BOE.5.002526. eCollection 2014 Aug 1.
9
Temporal focusing-based multiphoton excitation microscopy via digital micromirror device.基于时间聚焦的数字微镜器件多光子激发显微镜
Opt Lett. 2014 Jun 1;39(11):3134-7. doi: 10.1364/OL.39.003134.
10
Improvement of axial resolution and contrast in temporally focused widefield two-photon microscopy with structured light illumination.通过结构光照明在时间聚焦宽场双光子显微镜中提高轴向分辨率和对比度。
Biomed Opt Express. 2013 Jun 3;4(7):995-1005. doi: 10.1364/BOE.4.000995. Print 2013 Jul 1.
Zotero 插件