Benga G, Popescu O, Pop V I
Biosci Rep. 1985 Mar;5(3):223-8. doi: 10.1007/BF01119591.
A comparison of water diffusion in human erythrocytes and ghosts revealed a longer relaxation time in ghosts, corresponding to a decreased exchange rate. However, the diffusional permeability of ghosts was not significantly different from that of erythrocytes. The changes in water diffusion following exposure to p-chloromercuribenzene sulfonate (PCMBS) have been studied on ghosts suspended in isotonic solutions. It was found that a significant inhibitory effect of PCMBS on water diffusion occurred only after several minutes of incubation at 37 degrees C. No inhibition was noticed after short incubation at 0 degree C, as previously used in some labelling experiments. This indicates the location in the membrane interior of the SH groups involved in water diffusion across human erythrocyte membranes. The nuclear magnetic resonance (n.m.r.) method appears as a useful tool for studying changes in water diffusion in erythrocyte ghosts with the aim of locating the water channel.
对人体红细胞和血影中水扩散的比较显示，血影中的弛豫时间更长，这与交换速率降低相对应。然而，血影的扩散通透性与红细胞并无显著差异。在等渗溶液中悬浮的血影上，研究了暴露于对氯汞苯磺酸盐（PCMBS）后水扩散的变化。结果发现，PCMBS对水扩散的显著抑制作用仅在37℃孵育几分钟后才出现。如之前在一些标记实验中那样，在0℃短暂孵育后未观察到抑制作用。这表明参与水跨人体红细胞膜扩散的巯基位于膜内部。核磁共振（n.m.r.）方法似乎是一种有用的工具，可用于研究红细胞血影中水扩散的变化，以定位水通道。
