Benga G, Pop V I, Popescu O, Borza V
Department of Cell Biology, Faculty of Medicine, Medical and Pharmaceutical Institute Cluj-Napoca, Romania.
Biosci Rep. 1990 Feb;10(1):31-6. doi: 10.1007/BF01116848.
The characteristics of water diffusional permeability (P) of human red blood cells were studied on isolated erythrocytes by a doping nuclear magnetic resonance technique. In order to estimate the basal permeability the maximal inhibition of water diffusion was induced by exposure of red blood cells to p-chloromercuribenzene sulfonate (PCMBS) under various conditions (concentration, duration, temperature). The lowest values of P were around 0.7 X 10(-3) cm s-1 at 10 degrees C, 1.2 X 10(-3) cm s-1 at 15 degrees C, 1.4 X 10(-3) cm s-1 at 20 degrees C, 1.8 X 10(-3) cm s-1 at 25 degrees C, 2.1 X 10(-3) cm s-1 at 30 degrees C and 3.5 X 10(-3) cm s-1 at 37 degrees C. The mean value of the activation energy of water diffusion (Ea,d) was 25 kJ/mol for control and 43.7 kJ/mol for PCMBS--inhibited erythrocytes. The values of P and Ea,d obtained after induction of maximal inhibition of water diffusion by PCMBS can be taken as references for the basal permeability to water of the human red blood cell membrane.
采用掺杂核磁共振技术，在分离的红细胞上研究了人红细胞水扩散渗透率（P）的特性。为了估算基础渗透率，在各种条件（浓度、持续时间、温度）下，通过将红细胞暴露于对氯汞苯磺酸盐（PCMBS）来诱导水扩散的最大抑制。P的最低值在10℃时约为0.7×10⁻³ cm s⁻¹，15℃时为1.2×10⁻³ cm s⁻¹，20℃时为1.4×10⁻³ cm s⁻¹，25℃时为1.8×10⁻³ cm s⁻¹，30℃时为2.1×10⁻³ cm s⁻¹，37℃时为3.5×10⁻³ cm s⁻¹。对照情况下水扩散活化能（Ea,d）的平均值为25 kJ/mol，PCMBS抑制的红细胞为43.7 kJ/mol。PCMBS诱导水扩散最大抑制后获得的P和Ea,d值可作为人红细胞膜对水基础渗透率的参考。
