An Ning, Wang Li-juan, Lü Li-li, Fu Jian-nan, Wang Yu-fei, Zhao Jing-jing, Guo Huai-zhong
Yao Xue Xue Bao. 2016 Aug;51(8):1297-301.
We developed a new method for chiral separation of fourteen amino alcohols by nonaqueous capillary electrophoresis (NACE) with the D-(+)-gluconic acid δ-lactone-boric acid complex as chiral selector. In order to achieve good enantioseparation, the effects of D-(+)-gluconic acid δ-lactone and boric acid concentrations, triethylamine concentration, as well as capillary temperature were systematically investigated. The optimized conditions were identified as follows: an uncoated fused silica capillary of 50 μm ID with a total length (L(tot)) of 55 cm and an effective length (L(eff)) of 45 cm; 200 mmol·L(-1) D-(+)-gluconic acid δ-lactone, 80 mmol·L(-1) boric acid, and 57.4 mmol·L(-1) triethylamine in methanol; positive pressure injection at 2.9 psi for 2 s; capillary temperature, 25 ± 0.2 ℃; applied voltage, +15 k V; detection wavelength, 214 nm. Under the optimized conditions, a good chiral resolution was achieved in most of the tested drugs. This method provides a foundation for the development and application of new chiral selectors of polyhydroxy compound-boric acid complexes in chiral drugs analysis by NACE.
我们开发了一种新方法，以D-(+)-葡萄糖酸δ-内酯-硼酸络合物作为手性选择剂，通过非水毛细管电泳（NACE）对手性分离14种氨基醇。为了实现良好的对映体分离，系统地研究了D-(+)-葡萄糖酸δ-内酯和硼酸浓度、三乙胺浓度以及毛细管温度的影响。确定的优化条件如下：内径50μm的未涂层熔融石英毛细管，总长（L(tot)）为55cm，有效长度（L(eff)）为45cm；甲醇中含有200mmol·L(-1) D-(+)-葡萄糖酸δ-内酯、80mmol·L(-1)硼酸和57.4mmol·L(-1)三乙胺；在2.9psi下正压进样2s；毛细管温度25±0.2℃；施加电压+15kV；检测波长214nm。在优化条件下，大多数受试药物都实现了良好的手性拆分。该方法为多羟基化合物-硼酸络合物新型手性选择剂在NACE手性药物分析中的开发和应用奠定了基础。
