Effect of Curcumin on Characterization and Localization of Interleukin-13 and Tumor Necrosis Factor-alpha in Liver of Diabetic Rats.

OBJECTIVE

To localize and characterize inflammatory markers: interleukin-13 (IL-13) and Tumor necrosis factor-alpha (TNF-alpha) in recovery livers after curcumin supplementation in streptozotocin-induced diabetic rats.

MATERIAL AND METHOD

Induced diabetic male rats were achieved by streptozotocin intravenous injection (50 mg/kg BW). The rats were divided into three groups, control rat (C), diabetic rat (DM) and diabetic rat supplemented with curcumin (DMC) (200 mg/kg BW) that has been proposed for anti-inflammation and antioxidant activities. After 12 weeks of curcumin supplementation, liver tissues were collected and processed for hematoxylin & eosin staining and immunohistochemistry. The localization and characterization of IL-13 and TNF-alpha were investigated and compared among three groups in order to analyze the efficiency of curcumin in recovering liver tissues.

RESULTS

According DM group, high intensity of IL-13 and TNF-alpha were accumulated around central vein, along hepatic parenchyma, and at perivascular sinusoidal areas. In contrast, the characterization of IL-13 and TNF-alpha in DMC were attenuated. Then, the liver tissues were recovered and engaged by less severity sign of inflammations. Therefore, dietary curcumin might have efficacy to ameliorate diabetic complications in terms of controlling and modulating inflammatory parameters, including IL-13, and TNF-alpha.

CONCLUSION

Administration of curcumin successfully attenuated liver tissue by means of decreased inflammatory cytokine markers. The potential beneficial effects of curcumin have been shown to decline the inflammatory of liver tissue, concerning illustration of IL-13, and TNF-alpha. Therefore, the efficiency and achievement of curcumin might be applied to be an alternative therapeutic agent in diabetic liver.