Yang-en Sunantha, Petpiboolthai Hattaya, Khimmakthong Wipapan, Anupunpisit Vipavee
J Med Assoc Thai. 2016 Nov;99 Suppl 8:S118-S129.
To localize and characterize type I and type IV collagens in recovery livers after curcumin supplementation in streptozotocin-induced diabetic rats.
Induced diabetic rats were performed by streptozotocin injection (60 mg/kg BW). Male rats were organized into three groups, control rat (C), diabetic rat (DM) and diabetic rat supplemented with curcumin (DMC) (200 mg/kg BW). At 8 weeks, animals were sacrificed. The localization and characterization of type I and type IV collagens in liver’s cell and tissues were compared among C, DM and DMC groups by Sirius red and Immunohistochemical techniques, respectively.
Type I and type IV collagens might be the key mediators of liver tissue healing associated with various disorders, especially with inflammation and reorganization processes. Concerning diabetic experiments, increased type I collagen was intensively recognized at subendothelial area of central veins whereas weakly demonstrated at periportal triad and perisinusoidal areas. Conversely, the high intensity of distribution of type IV collagen was strongly revealed at periportal triad and perisinusoidal areas while the intensity was faintly presented at central veins. In addition, accumulation of type IV collagen also revealed perisinusoidal basement membrane which was characteristic of capillarization of sinusoids. However, the localization of type I and type IV collagens was reduced after curcumin supplement in DMC rats compared with DM rats, implying that the liver tissue reorganization has been developed forwards to normal morphology. Moreover, type I and type IV collagen might distinctively accomplish the liver tissue reorganization by different means of area-based characterization.
The potential beneficial effect of curcumin has been exhibited the tissue reorganization of diabetic liver tissues. The efficiency and achievement of curcumin might be applied to be an alternative therapeutic agent in diabetic hepatic pathology.
在链脲佐菌素诱导的糖尿病大鼠中，补充姜黄素后对恢复肝脏中的I型和IV型胶原蛋白进行定位和特性分析。
通过注射链脲佐菌素（60 mg/kg体重）诱导糖尿病大鼠。雄性大鼠分为三组，即对照大鼠（C组）、糖尿病大鼠（DM组）和补充姜黄素的糖尿病大鼠（DMC组）（200 mg/kg体重）。8周后处死动物。分别采用天狼星红染色和免疫组化技术比较C组、DM组和DMC组肝脏细胞和组织中I型和IV型胶原蛋白的定位和特性。
I型和IV型胶原蛋白可能是与各种疾病相关的肝组织愈合的关键介质，尤其是与炎症和重组过程相关。关于糖尿病实验，I型胶原蛋白增加在中央静脉内皮细胞下区域有强烈表现，而在门静脉三联体和窦周区域表现较弱。相反，IV型胶原蛋白在门静脉三联体和窦周区域有强烈的高强度分布，而在中央静脉区域强度较弱。此外，IV型胶原蛋白的积累还显示出窦周基底膜，这是肝血窦毛细血管化的特征。然而，与DM组相比，DMC组大鼠补充姜黄素后I型和IV型胶原蛋白的定位减少，这意味着肝组织重组已向正常形态发展。此外，I型和IV型胶原蛋白可能通过基于区域的不同特性分析方式独特地完成肝组织重组。
姜黄素的潜在有益作用已在糖尿病肝组织的组织重组中得到体现。姜黄素的有效性和成果可应用于糖尿病肝脏病理学的替代治疗药物。
