Department of Biological Sciences , University of Calgary , Calgary , Alberta , Canada T2N 1N4.
Department of Biology , University of Waterloo , Waterloo , Ontario , Canada N2L 3G.
Environ Sci Technol. 2018 Jul 17;52(14):7951-7961. doi: 10.1021/acs.est.8b01244. Epub 2018 Jul 3.
Salmonids are ecologically, economically, and culturally important fish species in North America, but whether contaminants in the environment play a role in their population decline is unclear. We tested the hypothesis that bisphenol A (BPA) deposition in eggs, mimicking a maternal transfer scenario, compromises the stress axis functioning and target tissues stress response in two generations of a model salmonid species, rainbow trout ( Oncorhynchus mykiss). Eggs were enriched with 0, 4, or 40 ng of BPA, fertilized, and reared in clean water for two generations. The fish were subjected to an acute stressor after a year in both generations to test their stress performances. Trout raised from BPA-enriched eggs showed impaired stressor-mediated plasma cortisol and lactate response in the F1 and F2 generations, respectively. Key genes involved in cortisol biosynthesis in the head kidney, as well as stress- and growth-related transcripts in the liver and muscle, were impacted either in the F1 and/or F2 generations. Our results underscore the long-term impact associated with BPA in eggs, mimicking a maternal transfer scenario, on the stress performance of trout in two generations. The results highlight the need for developing novel biomarkers to predict long-term and generational toxicities in salmonids.
鲑鱼是北美的一种具有重要生态、经济和文化意义的鱼类，但环境中的污染物是否会导致其数量下降尚不清楚。我们通过实验验证了以下假设：在鸡蛋中模拟母体传递的情况下，双酚 A（BPA）的沉积会影响两种鲑鱼模型物种（虹鳟鱼）的应激轴功能和靶组织的应激反应。我们用 0、4 或 40ng 的 BPA 对鸡蛋进行了富集，受精后在清洁水中进行了两代的繁殖。在经过一年的培育后，这两种鲑鱼都受到了急性应激源的刺激，以测试它们的应激表现。结果表明，与对照组相比，从 BPA 富集的鸡蛋中孵化出的鲑鱼在 F1 和 F2 代中表现出应激介导的血浆皮质醇和乳酸反应受损。头部肾脏中参与皮质醇生物合成的关键基因，以及肝脏和肌肉中与应激和生长相关的转录本，在 F1 和/或 F2 代中受到了影响。我们的研究结果强调了在鸡蛋中模拟母体传递的情况下，BPA 对鲑鱼两代应激表现的长期影响。这些结果凸显了开发新型生物标志物以预测鲑鱼长期和代际毒性的必要性。
