Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technická 5, 166 28 Prague, Czech Republic.
Department of Biotechnology, University of Chemistry and Technology Prague, Technická 5, 166 28 Prague, Czech Republic.
Virology. 2018 Aug;521:108-117. doi: 10.1016/j.virol.2018.06.001. Epub 2018 Jun 12.
Retrovirus assembly is driven mostly by Gag polyprotein oligomerization, which is mediated by inter and intra protein-protein interactions among its capsid (CA) domains. Mason-Pfizer monkey virus (M-PMV) CA contains three cysteines (C82, C193 and C213), where the latter two are highly conserved among most retroviruses. To determine the importance of these cysteines, we introduced mutations of these residues in both bacterial and proviral vectors and studied their impact on the M-PMV life cycle. These studies revealed that the presence of both conserved cysteines of M-PMV CA is necessary for both proper assembly and virus infectivity. Our findings suggest a crucial role of these cysteines in the formation of infectious mature particles.
逆转录病毒的组装主要由 gag 多聚蛋白的寡聚化驱动，这是由其衣壳 (CA) 结构域之间的蛋白-蛋白相互作用介导的。猴 Mason-Pfizer 病毒 (M-PMV) 的 CA 结构域包含三个半胱氨酸残基 (C82、C193 和 C213)，其中后两个在大多数逆转录病毒中高度保守。为了确定这些半胱氨酸的重要性，我们在细菌和前病毒载体中引入了这些残基的突变，并研究了它们对 M-PMV 生命周期的影响。这些研究表明，M-PMV CA 中这两个保守半胱氨酸的存在对于适当的组装和病毒感染性都是必需的。我们的发现表明这些半胱氨酸在形成感染性成熟颗粒中起着关键作用。
