Department of Molecular Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29 Yoshida-Shimoadachi-cho, Sakyo-ku, Kyoto, 606-8501, Japan.
Department of Clinical Pharmacology and Therapeutics, Kyoto University Hospital, 54 Shogoin-Kawahara-cho, Sakyo-ku, Kyoto, 606-8507, Japan.
J Pharmacol Sci. 2018 Jul;137(3):313-316. doi: 10.1016/j.jphs.2018.05.005. Epub 2018 May 31.
Neurotropin (NTP) is a Japanese analgesic agent for treating neuropathic pain; however, its method of action remains unclear. This study examined the effects of NTP on the activity of small dorsal root ganglion (DRG) neurons using whole-cell patch clamp recordings. After 3 days of treatment, NTP decreased current injection-induced firing activity of cultured DRG neurons by raising the current threshold for action potential generation. Additionally, NTP increased the sustained component of voltage-gated potassium (K) channel currents without affecting other K currents. These results suggest that NTP inhibits the firing activity of DRG neurons through augmentation of sustained K current.
神经妥乐平（NTP）是一种用于治疗神经性疼痛的日本镇痛剂；然而，其作用机制仍不清楚。本研究使用全细胞膜片钳记录技术，观察了 NTP 对小背根神经节（DRG）神经元活性的影响。治疗 3 天后，NTP 通过提高动作电位产生的电流阈值来降低培养的 DRG 神经元在电流注入诱导下的放电活动。此外，NTP 增加了电压门控钾（K）通道电流的持续成分，而不影响其他 K 电流。这些结果表明，NTP 通过增强持续的 K 电流来抑制 DRG 神经元的放电活动。
