Mutoh Tatsushi, Yamamoto Shuzo, Moriya Takahiro
Department of Aging Research and Geriatric Medicine, Institute of Development, Aging and Cancer, Tohoku University, Aoba-ku, Sendai 980-8575, Japan
Department of Pharmacology, School of Pharmaceutical Sciences, Ohu University, Koriyama, Fukushima 963-8611, Japan
Biomedicines. 2021 Jun 10;9(6):664. doi: 10.3390/biomedicines9060664.
Early brain injury (EBI) is closely linked to the development of delayed cerebral ischemia and poor outcomes after aneurysmal subarachnoid hemorrhage (SAH). This study aimed to evaluate the neuroprotective effect of neurotropin on EBI in a murine model of SAH. Twenty-four C57BL/6N mice were treated with intraperitoneal injections of either saline or 2.4 units of neurotropin at 1 h after SAH induction and for 3 days consecutively. SAH was created by an endovascular perforation method. In addition to the assessment of cerebral infarction and survival rate, motor and neurocognitive functions were also measured after SAH. Compared to the saline control group, the neurotropin group showed better recovery from locomotive and neurological declines after SAH. The neurotropin group also showed lower rates of post-SAH acute cerebral infarction and better memory and route-learning scores ( < 0.05). Meanwhile, there was no significant between-group differences in the overall mortality, hemodynamic parameters, or body weights. In conclusion, post-event treatment with neurotropin could be protective against EBI, lowering the incidence of ischemia and improving some motor and neurocognitive functions after SAH.
早期脑损伤（EBI）与动脉瘤性蛛网膜下腔出血（SAH）后迟发性脑缺血的发生及不良预后密切相关。本研究旨在评估神经妥乐平对SAH小鼠模型中EBI的神经保护作用。24只C57BL/6N小鼠在SAH诱导后1小时接受腹腔注射生理盐水或2.4单位神经妥乐平治疗，并连续治疗3天。采用血管内穿刺法制造SAH模型。除了评估脑梗死和生存率外，还在SAH后测量运动和神经认知功能。与生理盐水对照组相比，神经妥乐平组在SAH后运动和神经功能衰退的恢复情况更好。神经妥乐平组SAH后急性脑梗死发生率也较低，记忆和路径学习评分更高（P<0.05）。同时，在总体死亡率、血流动力学参数或体重方面，组间无显著差异。总之，SAH后使用神经妥乐平进行治疗可能对EBI具有保护作用，可降低缺血发生率，并改善SAH后的一些运动和神经认知功能。
