Magnuson N S, Perryman L E, Mason P H, Marta K M
Comp Biochem Physiol B. 1985;81(2):459-65. doi: 10.1016/0305-0491(85)90342-6.
A microassay requiring as few as 2 X 10(5) cells per assay was developed for systematic analysis of 9 purine enzymes in lymphocytes from equine peripheral blood, spleen, lymph node, thymus and bone marrow. The activities of adenosine deaminase (ADA), purine nucleoside phosphorylase (PNP), adenosine kinase (AK), deoxyadenosine kinase (dAK), deoxycytidine kinase (dCK), 5'-nucleotidase (5'-N), AMP deaminase, hypoxanthine-guanine phosphoribosyl transferase (HGPRT or HPRT), and adenine phosphoribosyl transferase (APRT) were measured by this microassay in lymphocytes from peripheral blood from four different breeds of horses (Arabian, Quarter Horse, Thoroughbred and Shetland Pony). There were no significant differences in the enzyme activities among the various breeds. Peripheral blood lymphocytes (PBL) from foals exhibited enzyme activities similar to those observed for adult animals. All lymphoid tissue contained similar levels of activity for each kinase (AK, dAK and dCK). Spleen had the highest activity for ADA, PNP, 5'-N, and HGPRT. The lowest activity for ADA, APRT, PNP and AMP deaminase was found in thymus. Enzymatic activities that varied the most among the tissue were 5'-N, ADA, APRT, HGPRT and AMP deaminase.
已开发出一种每次检测仅需2×10⁵个细胞的微量检测法，用于系统分析马外周血、脾脏、淋巴结、胸腺和骨髓淋巴细胞中的9种嘌呤酶。通过这种微量检测法，测定了四种不同品种马（阿拉伯马、夸特马、纯种马和设得兰矮种马）外周血淋巴细胞中腺苷脱氨酶（ADA）、嘌呤核苷磷酸化酶（PNP）、腺苷激酶（AK）、脱氧腺苷激酶（dAK）、脱氧胞苷激酶（dCK）、5'-核苷酸酶（5'-N）、AMP脱氨酶、次黄嘌呤-鸟嘌呤磷酸核糖转移酶（HGPRT或HPRT）和腺嘌呤磷酸核糖转移酶（APRT）的活性。不同品种之间的酶活性没有显著差异。幼驹的外周血淋巴细胞（PBL）表现出与成年动物相似的酶活性。所有淋巴组织中每种激酶（AK、dAK和dCK）的活性水平相似。脾脏中ADA、PNP、5'-N和HGPRT的活性最高。胸腺中ADA、APRT、PNP和AMP脱氨酶的活性最低。组织间变化最大的酶活性是5'-N、ADA、APRT、HGPRT和AMP脱氨酶。
