Narwal Vinay, Pundir C S
Department of Biochemistry, M.D.University, Rohtak, 124001, Haryana, India.
Department of Biochemistry, M.D.University, Rohtak, 124001, Haryana, India.
Anal Biochem. 2018 Aug 15;555:94-103. doi: 10.1016/j.ab.2018.06.005. Epub 2018 Jun 14.
Glycerol kinase (GK) and glycerol-3- phosphate oxidase (GPO) nanoparticles (NPs) were prepared, characterized and immobilized onto pencil graphite (PG) electrode to fabricate an improved amperometric glycerol biosensor (GKNPs/GPONPs/PGE). GKNPs/GPONPs/PGE worked in optimum conditions of pH 7.0, temperature 30 °C, at an applied potential of -0.3 V. The biosensor exhibited wide linear response in a concentration range of glycerol (0.01-45 mM) with detection limit 0.0001 μM. The biosensor revealed high sensitivity (7.24 μAmMcm), low response time (2.5s) and a good agreement with the standard enzymic colorimetric method with a correlation coefficient (R = 0.99). The evaluation study of biosensor offered a good analytical recovery of 98.73% when glycerol concentration was added to the sera sample. In addition, within and between batches study of working electrode showed coefficients of variation as 0.105% and 0.14%, respectively. The application of biosensor is performed in the serum of apparently healthy subject and patients affected by cardiogenic shock. There was a 20% loss in initial activity of biosensor after its regular use over a time period of 180 days, while being stored at 4 °C.
制备了甘油激酶（GK）和甘油-3-磷酸氧化酶（GPO）纳米颗粒（NPs），对其进行了表征，并将其固定在铅笔石墨（PG）电极上，以制造一种改进的安培型甘油生物传感器（GKNPs/GPONPs/PGE）。GKNPs/GPONPs/PGE在pH 7.0、温度30℃、施加电位为-0.3V的最佳条件下工作。该生物传感器在甘油浓度范围为0.01-45 mM时表现出宽线性响应，检测限为0.0001 μM。该生物传感器具有高灵敏度（7.24 μAmMcm）、低响应时间（2.5s），并且与标准酶比色法具有良好的一致性，相关系数（R = 0.99）。当向血清样品中添加甘油浓度时，生物传感器的评估研究显示出良好的分析回收率为98.73%。此外，工作电极的批内和批间研究显示变异系数分别为0.105%和0.14%。生物传感器的应用在表面健康受试者和心源性休克患者的血清中进行。在4℃储存180天后，生物传感器定期使用后初始活性损失了20%。
