Toxicology Division, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram 695 012, Kerala, India; Nanosciences Department, Laboratoire Interdisciplinaire Carnot de Bourgogne, UMR 6303, CNRS/Université Bourgogne Franche-Comté, 9 av. A. Savary BP 47 870, 21 078 Dijon, France.
Nanosciences Department, Laboratoire Interdisciplinaire Carnot de Bourgogne, UMR 6303, CNRS/Université Bourgogne Franche-Comté, 9 av. A. Savary BP 47 870, 21 078 Dijon, France.
Toxicol Appl Pharmacol. 2018 Aug 15;353:74-86. doi: 10.1016/j.taap.2018.06.013. Epub 2018 Jun 13.
Titanate nanotubes (TiONts) are promising agents for biomedical applications. Microglial activation and associated oxidative burst are major challenges in drug delivery applications across the brain. Here, TiONts were designed for drug delivery systems by functionalizing them with (3-aminopropyl) triethoxysilane (APTES), their interactions and biocompatibility were studied in vitro using murine microglial BV-2 cells. TiONts-APTES exposure resulted in increased ROS production and transient mitochondrial hyperpolarization. However, there was no indication of microglial proliferation in BV-2 cells as suggested by cell cycle analysis and morphology evaluation. The endocytosis as well as passive diffusion mediated TiONts-APTES internalization were proved by transmission electron microscopy (TEM) with and without amiloride, an endocytosis inhibiting agent. In addition, the TiONts-APTES exhibited good biocompatibility on microglial BV-2 cells as revealed by the plasma membrane integrity, lysosmal membrane integrity, morphology and viability analysis.
钛酸盐纳米管（TiONts）是一种很有前途的生物医学应用试剂。小胶质细胞激活和相关的氧化爆发是药物输送应用在整个大脑中面临的主要挑战。在这里，通过用（3-氨丙基）三乙氧基硅烷（APTES）对 TiONts 进行功能化设计，将其用作药物输送系统，并在体外使用小鼠小胶质细胞 BV-2 细胞研究其相互作用和生物相容性。TiONts-APTES 暴露会导致 ROS 产生增加和线粒体短暂超极化。然而，正如细胞周期分析和形态评估所表明的那样，BV-2 细胞中没有小胶质细胞增殖的迹象。通过使用和不使用阿米洛利（一种内吞作用抑制剂）的透射电子显微镜（TEM）证明了内吞作用以及由被动扩散介导的 TiONts-APTES 内化。此外，TiONts-APTES 在小胶质细胞 BV-2 细胞上表现出良好的生物相容性，这体现在质膜完整性、溶酶体膜完整性、形态和活力分析上。
