Caleffe Ronaldo Roberto Tait, de Oliveira Stefany Rodrigues, Gigliolli Adriana Aparecida Sinópolis, Ruvolo-Takasusuki Maria Claudia Colla, Conte Helio
Departament of Biotechnology, Genetics and Cell Biology of State University of Maringá, PR, Brazil.
Departament of Biotechnology, Genetics and Cell Biology of State University of Maringá, PR, Brazil.
Micron. 2018 Sep;112:55-62. doi: 10.1016/j.micron.2018.06.007. Epub 2018 Jun 8.
Larval therapy (LT) comprises the application of sterile Calliphoridae larvae for wound debridement, disinfection, and healing in humans and animals. Larval digestion plays a key role in LT, where the salivary glands and gut produce and secrete proteolytic and antimicrobial substances. The objective of this work was to bioprospect the salivary glands of Chrysomya megacephala (Fabricius, 1794) larvae, using ultrastructural, morphological, and histological observations, and the total protein electrophoretic profile. The salivary glands present a deferent duct, originating from the buccal cavity, which bifurcates into efferent ducts that insert through a slight dilatation to a pair of tubular-shaped tissues, united in the region of fat cells. Histologically, the secretion had protein characteristics. Cell cytoplasm presented numerous free ribosomes, autophagic vacuoles, spherical and elongated mitochondria, atypical Golgi complexes, and dilated rough endoplasmic reticulum. In the apical cytoplasm, secretory granules and microvilli secretions demonstrated intense protein synthesis, basal cytoplasm with trachea insertions, and numerous mitochondria. The present work described the ultrastructure and morphology of C. megacephala third instar salivary glands, confirming intense protein synthesis and the molecular weight of soluble proteins.
幼虫疗法（LT）包括将无菌丽蝇科幼虫应用于人和动物的伤口清创、消毒及愈合。幼虫消化在幼虫疗法中起关键作用，其唾液腺和肠道会产生并分泌蛋白水解和抗菌物质。本研究的目的是利用超微结构、形态学和组织学观察以及总蛋白电泳图谱，对大头金蝇（Chrysomya megacephala，Fabricius，1794）幼虫的唾液腺进行生物勘探。唾液腺有一条来自口腔的输送管，该输送管分支形成传出管，通过轻微扩张插入一对管状组织，这对管状组织在脂肪细胞区域相连。从组织学上看，分泌物具有蛋白质特征。细胞质中有大量游离核糖体、自噬泡、球形和细长形线粒体、非典型高尔基体复合体以及扩张的粗面内质网。在顶端细胞质中，分泌颗粒和微绒毛分泌物显示出强烈的蛋白质合成，基部细胞质有气管插入，且有大量线粒体。本研究描述了大头金蝇三龄幼虫唾液腺的超微结构和形态，证实了其强烈的蛋白质合成以及可溶性蛋白质的分子量。
