Akbari Hossein, Ghardashi Mostafa, Soleimani Alireza, Mohammadi Hanieh, Nikoueinejad Hassan
Department of Community Medicine, Kashan University of Medical Sciences, Kashan, Iran.
Student Research Committee, Kashan University of Medical Sciences, Kashan, Iran.
Iran J Allergy Asthma Immunol. 2018 Jun;17(3):258-264.
A subset of CD4+ T cells named T helper (Th)22 cells play some pathogenic roles in some autoimmune disorders such as type 1 diabetes (T1D). We aimed to study the correlation between the circulatory number of these cells and serum levels of its related cytokines with T1D as well as diabetic complications including metabolic control, atherosclerosis, and nephropathy. Fortynine patients with T1D and 43 healthy controls underwent the assessment of circulatory number of Th22 cells (by flow cytometry), serum level of Th22 related cytokines including Interleukin-22 (IL-22), Interleukin-10 (IL-10), Transforming growth factor-β (TGF-β), Tumor necrosis factor-α (TNF-α) (by ELISA) and carotid intima-media thickness (cIMT) measurement (by doppler ultrasonography). In addition, fasting blood and urine samples were taken to measure levels of hemoglobin A1C, lipid profile, cell blood count (CBC), serum and urine creatinine and urine protein in all participants. Th22 frequency and serum levels of IL-22 and TNF-α in patients were significantly higher than those in controls (p<0.001). Serum levels of IL-10 and TGF-β in healthy individuals were higher than those in patients (p<0.001). None of the Th22 related markers had a significant correlation with diabetic complications. There was only a significant effect of IL-22 on HbA1C variations. Th22 pathway has a significant correlation with T1D but not with its complications of cIMT and Urine Albumin/Creatinine Ratio (UACR). We report that Th22 pathway is not a good prognostic as well as diagnostic marker of early macrovascular complications in T1D.
一种名为辅助性T细胞22（Th22）细胞的CD4 + T细胞亚群在某些自身免疫性疾病如1型糖尿病（T1D）中发挥着一些致病作用。我们旨在研究这些细胞的循环数量及其相关细胞因子的血清水平与T1D以及糖尿病并发症（包括代谢控制、动脉粥样硬化和肾病）之间的相关性。49例T1D患者和43名健康对照者接受了Th22细胞循环数量评估（通过流式细胞术）、Th22相关细胞因子的血清水平评估，包括白细胞介素-22（IL-22）、白细胞介素-10（IL-10）、转化生长因子-β（TGF-β）、肿瘤坏死因子-α（TNF-α）（通过酶联免疫吸附测定法）以及颈动脉内膜中层厚度（cIMT）测量（通过多普勒超声检查）。此外，采集了所有参与者的空腹血液和尿液样本，以测量糖化血红蛋白A1C水平、血脂谱、血细胞计数（CBC）、血清和尿液肌酐以及尿蛋白。患者中Th22频率以及IL-22和TNF-α的血清水平显著高于对照组（p<0.001）。健康个体中IL-10和TGF-β的血清水平高于患者（p<0.001）。没有一种Th22相关标志物与糖尿病并发症有显著相关性。只有IL-22对糖化血红蛋白A1C变化有显著影响。Th22途径与T1D显著相关，但与cIMT和尿白蛋白/肌酐比值（UACR）等并发症无关。我们报告，Th22途径不是T1D早期大血管并发症的良好预后和诊断标志物。
