Clinic for Animal Reproduction, Faculty of Veterinary Medicine, Freie Universität Berlin, Koenigsweg 65, 14163 Berlin, Germany.
Department of Dairy Science, University of Wisconsin, Madison 53706.
J Dairy Sci. 2018 Sep;101(9):8566-8571. doi: 10.3168/jds.2017-14191. Epub 2018 Jun 13.
Incomplete luteal regression after treatment with a single dose of prostaglandin F during an Ovsynch protocol decreases fertility to timed artificial insemination (TAI). To increase the proportion of cows with complete luteal regression and subsequently pregnancy per artificial insemination (P/AI), an additional treatment with PGF 24 h after the first has been recommended. A systematic review of the literature and meta-analytical assessment were performed with the objective of evaluating the effects of adding a second PGF treatment during the Ovsynch protocol on luteal regression and reproductive performance in lactating dairy cows. Based on the heterogeneity among the experimental treatments, a fixed or a random effects meta-analysis was conducted. Reproductive outcomes of interest were luteal regression at the end of the Ovsynch protocol, and P/AI measured 32 to 39 d after TAI. Seven randomized controlled experiments from 6 published manuscripts including 5,356 cows with the primary objective to evaluate the effect of an additional treatment with PGF during the Ovsynch protocol on P/AI were used. Information regarding luteal regression at the end of the Ovsynch protocol was available for 1,856 cows. Adding a second PGF treatment on d 8 during the Ovsynch protocol increased the relative risk (RR) of complete luteal regression at the end of the Ovsynch protocol (RR = 1.14; 95% confidence interval = 1.10 to 1.17) using a fixed effects model and the RR for pregnancy (RR = 1.14; 95% confidence interval = 1.06 to 1.22) 32 d after TAI using a fixed effects model. No heterogeneity was observed among the 6 manuscripts regarding complete luteal regression and P/AI. In summary, there was a clear benefit of an additional PGF treatment during the Ovsynch protocol on luteal regression (+11.6 percentage units) and on P/AI (+4.6 percentage units).
在 Ovsynch 方案中单次使用前列腺素 F 治疗后黄体不完全退化会降低定时人工授精（TAI）的受胎率。为了增加黄体完全退化的牛的比例，并随后提高每人工授精（P/AI）的妊娠率，建议在第一次治疗后 24 小时再使用前列腺素 F 进行额外治疗。对文献进行了系统回顾和荟萃分析评估，目的是评估在 Ovsynch 方案中添加第二次前列腺素 F 治疗对泌乳奶牛黄体退化和繁殖性能的影响。根据实验处理之间的异质性，进行了固定或随机效应荟萃分析。感兴趣的生殖结果是 Ovsynch 方案结束时的黄体退化，以及 TAI 后 32 至 39 天的 P/AI。使用了来自 6 篇已发表文献的 7 项随机对照试验，这些试验的主要目的是评估在 Ovsynch 方案中添加第二次前列腺素 F 治疗对 P/AI 的影响，共有 5356 头奶牛。在 Ovsynch 方案结束时黄体退化的信息可用于 1856 头奶牛。在 Ovsynch 方案的第 8 天添加第二次前列腺素 F 治疗，使用固定效应模型时，增加了 Ovsynch 方案结束时完全黄体退化的相对风险（RR）（RR = 1.14；95%置信区间 = 1.10 至 1.17），使用固定效应模型时，TAI 后 32 天的妊娠率（RR = 1.14；95%置信区间 = 1.06 至 1.22）。关于完全黄体退化和 P/AI，6 篇文献之间没有观察到异质性。总之，在 Ovsynch 方案中进行额外的前列腺素 F 治疗对黄体退化（+11.6%）和 P/AI（+4.6%）有明显的益处。
