Gabor S, Ciugudeanu M, Surcel D
Environ Res. 1985 Aug;37(2):293-9. doi: 10.1016/0013-9351(85)90109-4.
Guinea pig peritoneal macrophages were incubated in vitro with various concentrations of selenium (as sodium selenite), quartz, and quartz + selenite. Cellular viability, adhesiveness, migration, and lipid peroxide content as parameters for cytotoxicity were studied. A dose-dependent cytotoxic response of macrophages to selenite was observed. Selenite concentrations of 10(-6), 10(-5), and 10(-4) M were shown to be nontoxic in the various test procedures. Quartz treatment of macrophages reduced cell viability, adhesiveness, and migration, and enhanced lipid peroxide release. Cotreatment of macrophages with nontoxic selenite concentrations suppressed significantly the cytotoxic effects induced by quartz and was effective in reducing the high rate of lipid peroxidation. The results provide support for the role of selenium in the stabilization of macrophage membrane damaged by quartz.
将豚鼠腹腔巨噬细胞与不同浓度的硒（以亚硒酸钠形式）、石英以及石英 + 亚硒酸钠进行体外培养。研究了细胞活力、黏附性、迁移能力以及脂质过氧化物含量作为细胞毒性的参数。观察到巨噬细胞对亚硒酸钠有剂量依赖性的细胞毒性反应。在各种测试程序中，10(-6)、10(-5) 和 10(-4) M 的亚硒酸钠浓度显示为无毒。用石英处理巨噬细胞会降低细胞活力、黏附性和迁移能力，并增加脂质过氧化物的释放。用无毒浓度的亚硒酸钠对巨噬细胞进行联合处理可显著抑制石英诱导的细胞毒性作用，并有效降低脂质过氧化的高发生率。这些结果为硒在稳定被石英损伤的巨噬细胞膜中的作用提供了支持。
