Druyts-Voets E, Yane F, Bosmans E, Colaert J, Desmyter J
Eur J Clin Microbiol. 1985 Jun;4(3):331-4. doi: 10.1007/BF02013662.
An outbreak of echovirus type 33-induced meningitis which occurred in Belgium in 1982 is reported. To identify the causative agent, titers of an early isolate were measured on a variety of cells in order to select the optimal cells. A human rhabdomyosarcoma cell line was found to be the substrate of choice due to its proficient isolation of the virus. This method of determining infectious titres is recommended for improving enterovirus isolation in other epidemics.
据报道，1982年在比利时发生了一起由33型埃可病毒引起的脑膜炎疫情。为确定病原体，对一份早期分离株在多种细胞上进行了滴度测定，以选择最佳细胞。发现人横纹肌肉瘤细胞系是首选底物，因为它能高效分离该病毒。推荐这种测定感染滴度的方法，以改进其他疫情中肠道病毒的分离。
