Mannhaupt G, Beyreuther K, Michaelis G
Eur J Biochem. 1985 Aug 1;150(3):435-9. doi: 10.1111/j.1432-1033.1985.tb09039.x.
The N-termini of four mitochondrial translation products, the var 1 protein, cytochrome b, and subunits I and III of cytochrome c oxidase have been characterized in Saccharomyces cerevisiae and compared with the known DNA sequences of the respective structural genes. The four mature proteins correspond to the predicted primary translation products and retain the formylated methionine residue. Thus, subunit II of cytochrome c oxidase studied previously [Pratje et al. (1983) EMBO J.2, 1049-1054] is so far the only mitochondrial translation product carrying a N-terminal-extended transient presequence in S. cerevisiae.
已对酿酒酵母中四种线粒体翻译产物（var 1蛋白、细胞色素b以及细胞色素c氧化酶的亚基I和III）的N端进行了表征，并与各自结构基因的已知DNA序列进行了比较。这四种成熟蛋白与预测的初级翻译产物相对应，并保留了甲酰化甲硫氨酸残基。因此，先前研究的细胞色素c氧化酶亚基II [普拉特杰等人（1983年），《欧洲分子生物学组织杂志》2，1049 - 1054]是迄今为止酿酒酵母中唯一带有N端延伸的瞬时前序列的线粒体翻译产物。
