College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agricultural University, Taian 271018, China.
College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agricultural University, Taian 271018, China.
Sci Total Environ. 2018 Nov 15;642:567-573. doi: 10.1016/j.scitotenv.2018.06.091. Epub 2018 Jun 14.
Potential toxicity to soil organisms by fluoxastrobin, a new strobilurin-type fungicide has drawn increasing attention. Thus, the present study investigated the subchronic toxicity induced by exposure to several concentrations (0, 0.1, 1.0, and 2.5 mg kg) of fluoxastrobin to earthworms on days 7, 14, 21, and 28. Biochemical indicators (e.g., reactive oxygen species (ROS) content, activities of antioxidase and detoxifying enzymes (superoxide dismutase, catalase, and glutathione S-transferase), lipid peroxidation (malonaldehyde) and degree of DNA damage) were measured. No earthworm deaths were observed during the entire experimental period. For ROS and malonaldehyde, the bioassay values of the three doses reached a maximum on day 21 and then decreased. For superoxide dismutase and glutathione S-transferase, the values increased with the exposure doses of 0.1 and 1.0 mg kg and then decreased. In contrast, the values for catalase were lower on days 7, 14, and 28 and greater on day 21 compared to those of the controls. In addition, the comet assay was more sensitive than other biomarkers, and the degree of DNA damage was dose and time -dependent.
新型甲氧基丙烯酸酯类杀菌剂氟唑菌酰胺对土壤生物的潜在毒性引起了越来越多的关注。因此，本研究在 7、14、21 和 28 天，通过暴露于氟唑菌酰胺的几个浓度（0、0.1、1.0 和 2.5mg/kg），调查了对蚯蚓的亚慢性毒性。测定了生化指标（如活性氧（ROS）含量、抗氧化酶和解毒酶（超氧化物歧化酶、过氧化氢酶和谷胱甘肽 S-转移酶）的活性、脂质过氧化（丙二醛）和 DNA 损伤程度）。在整个实验期间，未观察到蚯蚓死亡。对于 ROS 和丙二醛，三个剂量的生物测定值在第 21 天达到最大值，然后降低。对于超氧化物歧化酶和谷胱甘肽 S-转移酶，随着 0.1 和 1.0mg/kg 的暴露剂量的增加而增加，然后降低。相比之下，过氧化氢酶的值在第 7、14 和 28 天较低，而在第 21 天较高，与对照组相比。此外，彗星试验比其他生物标志物更敏感，并且 DNA 损伤程度具有剂量和时间依赖性。
