College of Resources and Environment, Shandong Agricultural University, Key Laboratory of Agricultural Environment in Universities of Shandong, 61 Daizong Road, Taian 271018, China.
College of Resources and Environment, Shandong Agricultural University, Key Laboratory of Agricultural Environment in Universities of Shandong, 61 Daizong Road, Taian 271018, China.
Sci Total Environ. 2021 Nov 25;797:149004. doi: 10.1016/j.scitotenv.2021.149004. Epub 2021 Jul 17.
Trifloxystrobin is a new type of fungicide, which is extensively used due to its excellent antifungal activity. In this study, oxidative stress and DNA damage induced by trifloxystrobin exposure was evaluated using Eisenia fetida at subchronic toxicity concentrations in artificial soil and brown soil (0.1-2.5 mg/kg). Throughout the exposure period (days 7, 28 and 56), six biochemical indicators including reactive oxygen species (ROS), antioxidant enzymes (SOD and CAT), glutathione S-transferase (GST), lipid peroxidation and DNA damage (8-hydroxydeoxyguanosine) were measured. In addition, the integrated biomarker response (IBR) index was calculated to make comparison of toxicological response between artificial and brown soils. Results indicated that trifloxystrobin can induce oxidative stress and DNA damage to earthworms with subchronic toxicity greater in brown soil compared to artificial soil as determined through integrated calculations for six biochemical indicators. Trifloxystrobin toxicological experiments in artificial soil may not accurately evaluate its toxicity in natural soil ecosystems, as the toxicity of trifloxystrobin to Eisenia fetida was underestimated.
三氟苯唑是一种新型杀菌剂，由于其优异的抗真菌活性而被广泛使用。本研究采用人工土壤和棕壤（0.1-2.5mg/kg），在亚慢性毒性浓度下，用赤子爱胜蚓评价三氟苯唑暴露引起的氧化应激和 DNA 损伤。在整个暴露期（第 7、28 和 56 天）内，测量了 6 种生化指标，包括活性氧（ROS）、抗氧化酶（SOD 和 CAT）、谷胱甘肽 S-转移酶（GST）、脂质过氧化和 DNA 损伤（8-羟基脱氧鸟苷）。此外，还计算了综合生物标志物响应（IBR）指数，以比较人工和棕壤之间的毒理反应。结果表明，三氟苯唑可诱导赤子爱胜蚓产生氧化应激和 DNA 损伤，亚慢性毒性在棕壤中大于人工土壤，这是通过对 6 种生化指标的综合计算得出的。在人工土壤中进行三氟苯唑毒性实验可能无法准确评估其在自然土壤生态系统中的毒性，因为三氟苯唑对赤子爱胜蚓的毒性被低估了。
