College of Environmental Science and Engineering, Hunan University and Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Ministry of Education, Changsha, 410082, Hunan, China.
Zhejiang Provincial Key Laboratory of Waste Treatment and Recycling, College of Environmental Science and Engineering, Zhejiang Gongshang University, Zhejiang, 310018, Hangzhou, China.
Appl Microbiol Biotechnol. 2018 Sep;102(17):7597-7610. doi: 10.1007/s00253-018-9143-x. Epub 2018 Jun 17.
A novel bionanomaterial comprising Saccharomyces cerevisiae (S. cerevisiae) and FeO nanoparticles encapsulated in a sodium alginate-polyvinyl alcohol (SA-PVA) matrix was synthesized for the efficient removal of atrazine from aqueous solutions. The effects of the operating parameters, nitrogen source, and glucose and Fe contents on atrazine removal were investigated, and the intermediates were detected by gas chromatography-mass spectrometry (GC-MS). In addition, the synthesized FeO particles were characterized by XRD, EDX, HR-TEM, FTIR, and hysteresis loops, and the bionanomaterial was characterized by SEM. The results showed that the maximum removal efficiency of 100% was achieved at 28 °C, a pH of 7.0, and 150 rpm with an initial atrazine concentration of 2.0 mg L and that the removal efficiency was still higher than 95.53% even when the initial atrazine concentration was 50 mg L. Biodegradation was demonstrated to be the dominant removal mechanism for atrazine because atrazine was consumed as the sole carbon source for S. cerevisiae. The results of GC-MS showed that dechlorination, dealkylation, deamination, isomerization, and mineralization occurred in the process of atrazine degradation, and thus, a new degradation pathway was proposed. These results indicated that this bionanomaterial has great potential for the bioremediation of atrazine-contaminated water.
一种新型的生物纳米材料，由酿酒酵母（Saccharomyces cerevisiae）和包埋在海藻酸钠-聚乙烯醇（SA-PVA）基质中的 FeO 纳米颗粒组成，用于从水溶液中高效去除莠去津。研究了操作参数、氮源以及葡萄糖和 Fe 含量对莠去津去除的影响，并通过气相色谱-质谱联用仪（GC-MS）检测了中间产物。此外，还通过 X 射线衍射（XRD）、能谱（EDX）、高分辨率透射电子显微镜（HR-TEM）、傅里叶变换红外光谱（FTIR）和磁滞回线对合成的 FeO 颗粒进行了表征，并通过扫描电子显微镜（SEM）对生物纳米材料进行了表征。结果表明，在 28°C、pH 值为 7.0、转速为 150rpm 的条件下，初始莠去津浓度为 2.0mg/L 时，去除效率最高可达 100%，即使初始莠去津浓度为 50mg/L 时，去除效率仍高于 95.53%。生物降解被证明是莠去津去除的主要机制，因为莠去津被用作酿酒酵母的唯一碳源而被消耗。GC-MS 的结果表明，在莠去津降解过程中发生了脱氯、脱烷基、脱氨、异构化和矿化作用，因此提出了一种新的降解途径。这些结果表明，该生物纳米材料在莠去津污染水的生物修复方面具有很大的潜力。
