来自珊瑚丛生软珊瑚的8R-脂氧合酶的表达
Expression of an 8R-Lipoxygenase From the Coral Plexaura homomalla.
作者信息
Gilbert Nathaniel C, Neau David B, Newcomer Marcia E
机构信息
Louisiana State University, Baton Rouge, LA, United States.
Cornell University, Northeastern Collaborative Access Team, Argonne National Laboratory, Argonne, IL, United States.
出版信息
Methods Enzymol. 2018;605:33-49. doi: 10.1016/bs.mie.2018.02.010. Epub 2018 Mar 28.
Abstract
Methods are presented for the use of the coral 8R-lipoxygenase from the Caribbean sea whip coral Plexaura homomalla as a model enzyme for structural studies of animal lipoxygenases. The 8R-lipoxygenase is remarkably stable and can be stored at 4°C for 3 months with virtually no loss of activity. In addition, an engineered "pseudo wild-type" enzyme is soluble in the absence of detergents, which helps facilitate the preparation of enzyme:substrate complexes.
摘要
本文介绍了使用来自加勒比海鞭珊瑚（Plexaura homomalla）的珊瑚8R-脂氧合酶作为动物脂氧合酶结构研究模型酶的方法。8R-脂氧合酶非常稳定，可在4°C下保存3个月，活性几乎没有损失。此外，一种工程化的“假野生型”酶在没有洗涤剂的情况下可溶，这有助于促进酶：底物复合物的制备。
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