来自珊瑚丛生软珊瑚的8R-脂氧合酶的表达
Expression of an 8R-Lipoxygenase From the Coral Plexaura homomalla.
作者信息
Gilbert Nathaniel C, Neau David B, Newcomer Marcia E
机构信息
Louisiana State University, Baton Rouge, LA, United States.
Cornell University, Northeastern Collaborative Access Team, Argonne National Laboratory, Argonne, IL, United States.
出版信息
Methods Enzymol. 2018;605:33-49. doi: 10.1016/bs.mie.2018.02.010. Epub 2018 Mar 28.
Abstract
Methods are presented for the use of the coral 8R-lipoxygenase from the Caribbean sea whip coral Plexaura homomalla as a model enzyme for structural studies of animal lipoxygenases. The 8R-lipoxygenase is remarkably stable and can be stored at 4°C for 3 months with virtually no loss of activity. In addition, an engineered "pseudo wild-type" enzyme is soluble in the absence of detergents, which helps facilitate the preparation of enzyme:substrate complexes.
摘要
本文介绍了使用来自加勒比海鞭珊瑚（Plexaura homomalla）的珊瑚8R-脂氧合酶作为动物脂氧合酶结构研究模型酶的方法。8R-脂氧合酶非常稳定，可在4°C下保存3个月，活性几乎没有损失。此外，一种工程化的“假野生型”酶在没有洗涤剂的情况下可溶，这有助于促进酶：底物复合物的制备。
相似文献
1
Expression of an 8R-Lipoxygenase From the Coral Plexaura homomalla.来自珊瑚丛生软珊瑚的8R-脂氧合酶的表达
Methods Enzymol. 2018;605:33-49. doi: 10.1016/bs.mie.2018.02.010. Epub 2018 Mar 28.
2
Catalase-Related Allene Oxide Synthase, on a Biosynthetic Route to Fatty Acid Cyclopentenones: Expression and Assay of the Enzyme and Preparation of the 8R-HPETE Substrate.过氧化氢酶相关丙二烯氧化物合酶：脂肪酸环戊烯酮生物合成途径中的酶表达、测定及8R-HPETE底物的制备
Methods Enzymol. 2018;605:51-68. doi: 10.1016/bs.mie.2018.02.019. Epub 2018 May 2.
3
Insights from the X-ray crystal structure of coral 8R-lipoxygenase: calcium activation via a C2-like domain and a structural basis of product chirality.珊瑚8R-脂氧合酶的X射线晶体结构解析：通过类C2结构域实现钙激活及产物手性的结构基础
J Biol Chem. 2005 Nov 25;280(47):39545-52. doi: 10.1074/jbc.M506675200. Epub 2005 Sep 14.
4
Purification and molecular cloning of an 8R-lipoxygenase from the coral Plexaura homomalla reveal the related primary structures of R- and S-lipoxygenases.从珊瑚虫软珊瑚中纯化并分子克隆一种8R-脂氧合酶，揭示了R-和S-脂氧合酶相关的一级结构。
J Biol Chem. 1996 Aug 23;271(34):20949-57. doi: 10.1074/jbc.271.34.20949.
5
8R-Lipoxygenase-catalyzed synthesis of a prominent cis-epoxyalcohol from dihomo-γ-linolenic acid: a distinctive transformation compared with S-lipoxygenases.8R-脂氧合酶催化二高γ-亚麻酸生成主要的顺式环氧醇：与 S-脂氧合酶相比的独特转化。
J Lipid Res. 2012 Feb;53(2):292-9. doi: 10.1194/jlr.M022863. Epub 2011 Dec 10.
6
Biotransformation of docosahexaenoic acid into 10R,17S-dihydroxydocosahexaenoic acid as protectin DX 10-epimer by serial reactions of arachidonate 8R- and 15S-lipoxygenases.二十二碳六烯酸通过花生四烯酸 8R-和 15S-脂加氧酶的连续反应转化为 10R,17S-二羟基二十二碳六烯酸作为保护素 DX10-差向异构体。
World J Microbiol Biotechnol. 2024 May 29;40(7):219. doi: 10.1007/s11274-024-04032-9.
7
Applying internal coordinate mechanics to model the interactions between 8R-lipoxygenase and its substrate.运用内坐标力学模型研究 8R-脂氧合酶与其底物的相互作用。
BMC Bioinformatics. 2010 Oct 7;11 Suppl 6(Suppl 6):S2. doi: 10.1186/1471-2105-11-S6-S2.
8
Identification and characterization of an arachidonate 11R-lipoxygenase.一种花生四烯酸11R-脂氧合酶的鉴定与特性分析
Arch Biochem Biophys. 2006 Jan 1;445(1):147-55. doi: 10.1016/j.abb.2005.10.023. Epub 2005 Nov 15.
9
10
Identification of amino acid determinants of the positional specificity of mouse 8S-lipoxygenase and human 15S-lipoxygenase-2.小鼠8S-脂氧合酶和人15S-脂氧合酶-2位置特异性的氨基酸决定簇的鉴定。
J Biol Chem. 2000 Jan 14;275(2):1287-93. doi: 10.1074/jbc.275.2.1287.
引用本文的文献
1
Investigating membrane-binding properties of lipoxygenases using surface plasmon resonance.利用表面等离子体共振研究脂氧合酶的膜结合特性。
Biochem Biophys Res Commun. 2023 Aug 30;670:47-54. doi: 10.1016/j.bbrc.2023.05.066. Epub 2023 May 27.
2
Revising the structure of a new eicosanoid from human platelets to 8,9-11,12-diepoxy-13-hydroxyeicosadienoic acid.从人血小板中对新型类二十烷酸的结构进行修正得到 8,9-11,12-环氧-13-羟二十碳二烯酸。
J Biol Chem. 2019 Jun 7;294(23):9225-9238. doi: 10.1074/jbc.RA119.008915. Epub 2019 May 6.
3
Stabilization and improved activity of arachidonate 11-lipoxygenase from proteobacterium .从产碱菌中稳定化和提高花生四烯酸 11-脂氧合酶的活性。
J Lipid Res. 2018 Nov;59(11):2153-2163. doi: 10.1194/jlr.M088823. Epub 2018 Sep 26.
4
Catalase-Related Allene Oxide Synthase, on a Biosynthetic Route to Fatty Acid Cyclopentenones: Expression and Assay of the Enzyme and Preparation of the 8R-HPETE Substrate.过氧化氢酶相关丙二烯氧化物合酶：脂肪酸环戊烯酮生物合成途径中的酶表达、测定及8R-HPETE底物的制备
Methods Enzymol. 2018;605:51-68. doi: 10.1016/bs.mie.2018.02.019. Epub 2018 May 2.
本文引用的文献
1
Investigation of calcium-dependent activity and conformational dynamics of zebra fish 12-lipoxygenase.斑马鱼 12-脂氧合酶钙依赖性活性与构象动力学研究。
Biochim Biophys Acta Gen Subj. 2017 Aug;1861(8):2099-2111. doi: 10.1016/j.bbagen.2017.05.015. Epub 2017 May 19.
2
Protein stability: a crystallographer's perspective.蛋白质稳定性：晶体学家的视角
Acta Crystallogr F Struct Biol Commun. 2016 Feb;72(Pt 2):72-95. doi: 10.1107/S2053230X15024619. Epub 2016 Jan 26.
3
The structural basis for specificity in lipoxygenase catalysis.脂氧合酶催化特异性的结构基础。
Protein Sci. 2015 Mar;24(3):298-309. doi: 10.1002/pro.2626. Epub 2015 Jan 13.
4
Crystal structure of a lipoxygenase in complex with substrate: the arachidonic acid-binding site of 8R-lipoxygenase.与底物结合的脂氧合酶的晶体结构：8R-脂氧合酶的花生四烯酸结合位点
J Biol Chem. 2014 Nov 14;289(46):31905-31913. doi: 10.1074/jbc.M114.599662. Epub 2014 Sep 17.
5
Crystal structure of 12-lipoxygenase catalytic-domain-inhibitor complex identifies a substrate-binding channel for catalysis.12-脂氧合酶催化结构域-抑制剂复合物的晶体结构确定了催化的底物结合通道。
Structure. 2012 Sep 5;20(9):1490-7. doi: 10.1016/j.str.2012.06.003. Epub 2012 Jul 12.
6
The structure of human 5-lipoxygenase.人 5-脂氧合酶的结构。
Science. 2011 Jan 14;331(6014):217-9. doi: 10.1126/science.1197203.
7
Lipoxygenases - Structure and reaction mechanism.脂氧合酶-结构和反应机制。
Phytochemistry. 2009 Sep;70(13-14):1504-10. doi: 10.1016/j.phytochem.2009.05.008. Epub 2009 Sep 18.
8
The 1.85 A structure of an 8R-lipoxygenase suggests a general model for lipoxygenase product specificity.8R-脂氧合酶的1.85埃结构揭示了脂氧合酶产物特异性的一般模型。
Biochemistry. 2009 Aug 25;48(33):7906-15. doi: 10.1021/bi900084m.
9
A covalent linker allows for membrane targeting of an oxylipin biosynthetic complex.共价连接子可实现氧化脂质生物合成复合物的膜靶向。
Biochemistry. 2008 Oct 7;47(40):10665-76. doi: 10.1021/bi800751p. Epub 2008 Sep 12.
10
Improving protein crystal quality by selective removal of a Ca(2+)-dependent membrane-insertion loop.通过选择性去除Ca(2+)依赖性膜插入环来提高蛋白质晶体质量。
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Nov 1;63(Pt 11):972-5. doi: 10.1107/S1744309107050993. Epub 2007 Oct 24.
Zotero 插件