Li Haihong, Chen Liyun, Zhang Mingjun, Xie Sitian, Cheng Liuhanghang
Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou, 515041, Guangdong Province, China; Research Center for Translational Medicine, Shantou University Medical College, North Dongxia Road, Shantou, 515041, Guangdong Province, China.
Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou, 515041, Guangdong Province, China.
Acta Histochem. 2018 Aug;120(6):520-524. doi: 10.1016/j.acthis.2018.06.003. Epub 2018 Jun 14.
Previously studies showed that Forkhead transcription factor A1 (FoxA1) was associated with sweat secretion. To investigate the expression and localization of FoxA1 in the three-dimensional (3D) reconstructed eccrine sweat glands, eccrine sweat gland cells were transplanted subcutaneously into nude mice with Matrigel, and at 2, 3, 4, 5, 6, 8, 10 and 12 weeks post-transplantation, the reconstructed eccrine sweat glands were removed and immunostained for FoxA1 and co-immunostained for FoxA1 and eccrine sweat markers, K7, carbonic anhydrase II (CA Ⅱ), gross cystic disease fluid protein-15 (GCDFP-15) and α-smooth muscle actin (α-SMA), and FoxA1 and sweat secretion-related proteins, Na-K-ATPase α and Na-K-2Cl- cotransporter 1 (NKCC1). The results showed that FoxA1-positive cells weren't detected until 3 weeks post-implantation, a time point of the differntiation of secretory coil-like structures. From the fourth week on, the number of FoxA1-positive cells increased and thereafter maintained at a high number. Double immunofluorescence staining showed that FoxA1-positive cells co-expressed dark cell marker GCDFP-15 and myoepithelial cell marker α-SMA, as well as secretion-related proteins, Na-K-ATPase α and NKCC1 in both the native and reconstructed eccrine sweat glands. In conclusion, FoxA1 might be related to the development and differentiation of secretory coil-like structures, as well as the secretory function of the 3D reconstructed eccrine sweat glands.
先前的研究表明，叉头转录因子A1（FoxA1）与汗液分泌有关。为了研究FoxA1在三维（3D）重建的外泌汗腺中的表达和定位，将外泌汗腺细胞与基质胶一起皮下移植到裸鼠体内，在移植后2、3、4、5、6、8、10和12周，取出重建的外泌汗腺，对FoxA1进行免疫染色，并对FoxA1与外泌汗腺标志物K7、碳酸酐酶II（CAⅡ）、大囊性病液蛋白-15（GCDFP-15）和α-平滑肌肌动蛋白（α-SMA）进行共免疫染色，以及对FoxA1与汗液分泌相关蛋白钠钾ATP酶α和钠钾2氯协同转运蛋白1（NKCC1）进行共免疫染色。结果显示，直到植入后3周才检测到FoxA1阳性细胞，这是分泌盘曲样结构分化的时间点。从第4周开始，FoxA1阳性细胞数量增加，此后维持在较高水平。双重免疫荧光染色显示，在天然和重建的外泌汗腺中，FoxA1阳性细胞共表达暗细胞标志物GCDFP-15和肌上皮细胞标志物α-SMA，以及分泌相关蛋白钠钾ATP酶α和NKCC1。总之，FoxA1可能与分泌盘曲样结构的发育和分化以及3D重建外泌汗腺的分泌功能有关。
