Department of Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou, 515041, Guangdong, China.
J Mol Histol. 2018 Dec;49(6):567-575. doi: 10.1007/s10735-018-9795-y. Epub 2018 Sep 20.
Epidermal basal cells invaginate into the dermis to form sweat ducts, which then grow downwards further to form secretory coils during the ontogenesis of eccrine sweat glands, but the time course of differentiation of different cell types in 3D-reconstructed eccrine sweat glands remain unclear. In this study, secretory cell-specific marker K7, clear secretory cell-specific marker CA II, dark secretory cell-specific marker GCDFP-15, myoepithelial cell-specific marker α-SMA, inner duct cell-specific marker S100P and outer duct cell-specific marker S100A2 were detected by immunofluorescence staining. The results showed that S100P and S100A2 were first detected at 2 weeks post implantation, K7 and α-SMA at 3 weeks, and GCDFP-15 and CA II at 4 weeks. The differentiation of ducts preceded secretory coils in 3D-reconstructed eccrine sweat glands. After 8 weeks post implantation, the distribution of these markers in 3D-reconstructed eccrine sweat glands was similar to that in native ones, and the percentage of the 3D-reconstructed glands expressing these markers maintained steady. We conclude that although the 3D-reconstructed and native eccrine sweat glands originated from different cells, the differentiation of different cell types in 3D-reconstructed eccrine sweat glands parallels the sequence observed during embryonic development.
表皮基底层向内凹陷形成真皮，形成汗腺导管，然后在汗腺的个体发生过程中向下生长形成分泌圈，但三维重建的汗腺中不同细胞类型的分化时间过程仍不清楚。在这项研究中，通过免疫荧光染色检测了分泌细胞特异性标志物 K7、清晰分泌细胞特异性标志物 CA II、暗分泌细胞特异性标志物 GCDFP-15、肌上皮细胞特异性标志物 α-SMA、内导管细胞特异性标志物 S100P 和外导管细胞特异性标志物 S100A2。结果表明，S100P 和 S100A2 最早在植入后 2 周检测到，K7 和 α-SMA 在 3 周，GCDFP-15 和 CA II 在 4 周。导管的分化先于三维重建的汗腺中的分泌圈。植入后 8 周，这些标志物在三维重建的汗腺中的分布与天然汗腺相似，表达这些标志物的三维重建腺体的百分比保持稳定。我们得出结论，尽管三维重建的和天然的汗腺来源于不同的细胞，但三维重建的汗腺中不同细胞类型的分化与胚胎发育过程中观察到的顺序相似。
