Palomino J Manuel, Jones Lori, Vanhanen Tom, Mastromonaco Gabriela F, Busato Rachel, Adams Gregg P
Department of Biomedical Sciences, University of Guelph, Guelph, Ontario N1G 2W1 (Palomino); Arriba Linea alpacas, Uxbridge, Ontario (Jones, Vanhanen); Reproductive Physiology, Toronto Zoo, Ontario (Mastromonaco); Port Perry Veterinary Services, Port Perry, Ontario (Busato); Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, Saskatchewan (Adams, Palomino).
Can Vet J. 2018 Jun;59(6):631-634.
This study evaluated the feasibility of using an embryo transfer protocol in an alpaca farm in Canada. Alpaca donors and recipients were synchronized with 2 doses of gonadotrophin-releasing hormone (GnRH), 12 days apart. In donors ( = 5), superstimulation was induced with follicle stimulating hormone (FSH) given daily (40 mg) for 5 days beginning 2 days after the second GnRH treatment. Cloprostenol was given on the last day of FSH, the donors were bred 2 days later, embryos were collected 7 days after breeding. In recipients ( = 8), the second dose of GnRH was given the day before donor mating, and embryos were transferred on the day of donor collection. On average (± SEM), 5.2 ± 1.4 were detected and 2.5 ± 1.2 transferable embryos were collected in the donors. A mature was detected in 6/8 synchronized recipients and a single embryo was transferred to each. One recipient alpaca became pregnant and delivered a healthy baby 349 days after embryo transfer. This is the first report of successful embryo transfer in alpacas in Canada.
本研究评估了在加拿大一个羊驼养殖场使用胚胎移植方案的可行性。羊驼供体和受体用两剂促性腺激素释放激素（GnRH）进行同期发情处理，间隔12天。在供体（n = 5）中，在第二次GnRH处理后2天开始，每天给予促卵泡素（FSH，40毫克），持续5天，以诱导超数排卵。在FSH处理的最后一天给予氯前列醇，供体在2天后配种，配种后7天采集胚胎。在受体（n = 8）中，在供体配种前一天给予第二剂GnRH，并在供体采集胚胎当天进行胚胎移植。供体平均（±标准误）检测到5.2±1.4个卵泡，采集到2.5±1.2个可移植胚胎。在8个同期发情的受体中有6个检测到成熟卵泡，每个受体移植单个胚胎。一只受体羊驼怀孕，并在胚胎移植后349天产下一只健康幼崽。这是加拿大关于羊驼胚胎移植成功的首次报道。
