Okada A, Shin T, Dworkin-Rastl E, Dworkin M B, Zubay G
Differentiation. 1985;29(1):14-9. doi: 10.1111/j.1432-0436.1985.tb00286.x.
A study was undertaken to test for the occurrence of DNA rearrangements or amplifications during embryonic development in Xenopus laevis. DNA isolated from testes and liver was digested with four restriction enzymes, separated on agarose gels, transferred to nitrocellulose, and hybridized with over 50 cloned cDNA probes generated from embryonic poly (A)+ RNA. No qualitative or quantitative differences were detectable in the DNA hybridization patterns of testes and liver DNA, suggesting that, at least during liver development, selective amplifications or rearrangements occur rarely if at all. In the course of this investigation a wide range of restriction-site polymorphisms for different genes was observed. While some genes showed little polymorphism among different animals, several genes showed considerable polymorphism, involving changes in several restriction enzyme sites. These complex polymorphisms could be the result of gene rearrangements that occur occasionally during the course of sexual reproduction rather than during development.
开展了一项研究，以检测非洲爪蟾胚胎发育过程中DNA重排或扩增的发生情况。从睾丸和肝脏中分离出的DNA用四种限制性内切酶消化，在琼脂糖凝胶上分离，转移到硝酸纤维素膜上，并用从胚胎多聚腺苷酸加尾RNA生成的50多个克隆cDNA探针进行杂交。在睾丸和肝脏DNA的DNA杂交模式中未检测到定性或定量差异，这表明，至少在肝脏发育过程中，选择性扩增或重排极少发生，甚至根本不发生。在这项研究过程中，观察到了不同基因的广泛限制性位点多态性。虽然一些基因在不同动物之间显示出很少的多态性，但有几个基因显示出相当大的多态性，涉及几个限制性酶切位点的变化。这些复杂的多态性可能是有性生殖过程中偶尔发生的基因重排的结果，而不是发育过程中的结果。
