Beckmann J S, Kashi Y, Hallerman E M, Nave A, Soller M
Anim Genet. 1986;17(1):25-38. doi: 10.1111/j.1365-2052.1986.tb03185.x.
Israeli Holstein-Friesian dairy bulls were screened for restriction fragment length polymorphisms by hybridizing cloned DNA probes for bovine growth hormone, for chymosin, and for rat muscle beta-actin to restriction endonuclease-digested DNA immobilized on nitrocellulose filters. The population proved to be polymorphic at the growth hormone locus, with evidence consistent with the phenotypes being inherited in allelic fashion. A low level of polymorphism was also observed at one of the beta-actin gene family loci. The chymosin locus was monomorphic with the restriction enzymes utilized. The results illustrate the power of restriction fragment length polymorphism methodology in visualizing genetic variability in dairy cattle populations.
通过将牛生长激素、凝乳酶和大鼠肌肉β-肌动蛋白的克隆DNA探针与固定在硝酸纤维素滤膜上的经限制性内切酶消化的DNA杂交，对以色列荷斯坦-弗里生奶牛公牛进行了限制性片段长度多态性筛查。结果证明，该群体在生长激素基因座上具有多态性，有证据表明其表型是以等位基因方式遗传的。在β-肌动蛋白基因家族的一个基因座上也观察到了低水平的多态性。利用这些限制性内切酶时，凝乳酶基因座是单态的。这些结果说明了限制性片段长度多态性方法在观察奶牛群体遗传变异性方面的作用。
