Campantico E, Guastalla A, Patriarca E
Gen Comp Endocrinol. 1985 Aug;59(2):192-8. doi: 10.1016/0016-6480(85)90369-7.
The indirect immunofluorescence procedure was used to localize ACTH-producing cells in the pituitary distal lobe from Hyla arborea adult specimens; the antiserum employed was rabbit anti-synthetic ACTH (1-24) conjugated with BSA. Immunohistochemical staining was suppressed by solid-phase absorption of the anti-ACTH with the specific antigen. In the distal lobe one cell population is immunoreactive; these cells are predominantly localized in the anterior half, both ventrally and dorsally; they exclusively correspond to type-3 basophils. In the intermediate lobe the total cell population is immunoreactive. Parallel inspections carried out on the pituitary of Xenopus laevis specimens gave results fully consistent with those reported by other authors.
采用间接免疫荧光法对成年树蛙垂体远侧叶中产生促肾上腺皮质激素（ACTH）的细胞进行定位；所用抗血清为与牛血清白蛋白（BSA）偶联的兔抗合成ACTH（1-24）。抗ACTH与特异性抗原的固相吸收抑制了免疫组织化学染色。在远侧叶中，有一群细胞具有免疫反应性；这些细胞主要位于前半部分，腹侧和背侧均有；它们仅对应于3型嗜碱性细胞。在中间叶，所有细胞群体都具有免疫反应性。对非洲爪蟾垂体进行的平行检查结果与其他作者报道的结果完全一致。
