Puro Kekungu-U, Bhattacharjee Uttaran, Baruah Samprity, Sen Arnab, Das Samir, Ghatak Sandeep, Doley Sunil, Sanjukta Rajkumari, Shakuntala Ingudam
1Animal Health Division, ICAR-Research Complex for North Eastern Hill Region, Umiam, Meghalaya 793103 India.
2Poultry Section, Livestock Production Division, ICAR-Research Complex for North Eastern Hill Region, Umiam, Meghalaya 793103 India.
Virusdisease. 2018 Jun;29(2):167-172. doi: 10.1007/s13337-018-0448-2. Epub 2018 Apr 3.
The aim of the present study was to characterize the virus from the lesions and histopathology of organs associated with mortality in Kuroiler (dual purpose variety of poultry developed and marketed by Keggfarms Pvt. Ltd, India) birds suspected of Marek's disease. Among 1047 birds from two farms of different location with 5.5 and 34% mortality, two types of lesion were observed in post mortem examination; tumors in vital organs-liver, spleen, kidney, lung and ovaries and generalized small nodular tumour in the abdominal cavity. Molecular characterization based on detection of gene showed the presence of Marek's disease virus (MDV) from tissues and cell culture adapted isolates in Madin Darby Canine Kidney cell lines. Histopathological examination revealed multinucleated immature lymphoid cells infiltration in the organs. Phylogenetic analysis of the isolates based on gene showed the isolates belongs to cluster I genotype of MDV. This is for the first time the MDV virus is characterized from an outbreak in the poultry flock in farmer's field affecting production in Meghalaya state of North east India.
本研究的目的是从疑似患马立克氏病的库罗iler鸡（印度Keggfarms Pvt. Ltd开发和销售的两用家禽品种）的病变以及与死亡相关的器官组织病理学中鉴定病毒。在来自两个不同地点农场的1047只鸡中，死亡率分别为5.5%和34%，死后检查观察到两种类型的病变；重要器官（肝脏、脾脏、肾脏、肺和卵巢）出现肿瘤，腹腔出现广泛性小结节肿瘤。基于基因检测的分子鉴定表明，从组织和适应细胞培养的分离株中检测到马立克氏病病毒（MDV），这些分离株来自犬肾细胞系。组织病理学检查显示，器官中有多核未成熟淋巴细胞浸润。基于基因对分离株进行的系统发育分析表明，这些分离株属于MDV的I簇基因型。这是首次从印度东北部梅加拉亚邦农民田间影响生产的家禽群疫情中鉴定出MDV病毒。
