基于2015 - 2018年诊断检查结果的波兰马立克氏病发病情况
Occurrence of Marek's Disease in Poland on the Basis of Diagnostic Examination in 2015-2018.
作者信息
Kozdruń Wojciech, Styś-Fijoł Natalia, Czekaj Hanna, Piekarska Karolina, Niczyporuk Jowita Samanta, Stolarek Agnieszka
机构信息
Department of Poultry Viral Diseases National Veterinary Research Institute, 24-100 Puławy, Poland.
出版信息
J Vet Res. 2020 Dec 1;64(4):503-507. doi: 10.2478/jvetres-2020-0079. eCollection 2020 Dec.
INTRODUCTION
Marek's disease (MD) is a tumourous disease caused by Marek's disease virus (MDV) and most commonly described in poultry. The aim of the study was to determine the occurrence of Marek's disease virus infections in Poland and analyse clinical cases in the years 2015-2018.
MATERIAL AND METHODS
The birds for diagnostic examination originated from 71 poultry flocks of various types of production. Birds were subjected to anatomopathological examination post mortem, during which liver and spleen sections and other pathologically changed internal organs were taken. These sections were homogenised with generally accepted methods, then total DNA was isolated and amplified with a real-time PCR. A pair of primers complementary to the MDV genome region encoding the gene were used.
RESULTS
MDV infection was found predominantly in broiler chicken flocks (69.01%), and also in layer breeder (9.85%) and commercial layer flocks (7.04% each).
CONCLUSION
The results of research conducted in the years 2015-2018 clearly indicate that the problem of MDV infections is still current.
引言
马立克氏病（MD）是一种由马立克氏病病毒（MDV）引起的肿瘤性疾病，最常见于家禽。本研究的目的是确定波兰马立克氏病病毒感染的发生率，并分析2015 - 2018年期间的临床病例。
材料与方法
用于诊断检查的家禽来自71个不同生产类型的家禽群。家禽在死后进行解剖病理学检查，在此期间采集肝脏和脾脏切片以及其他发生病理变化的内部器官。这些切片采用普遍认可的方法进行匀浆，然后分离总DNA并通过实时PCR进行扩增。使用了一对与编码该基因的MDV基因组区域互补的引物。
结果
MDV感染主要在肉鸡群中发现（69.01%），也存在于蛋种鸡群（9.85%）和商品蛋鸡群中（各7.04%）。
结论
2015 - 2018年进行的研究结果清楚地表明，MDV感染问题仍然存在。
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