Hink W F, Butz J J
In Vitro Cell Dev Biol. 1985 Jun;21(6):333-9. doi: 10.1007/BF02691581.
Venom from the ant Pseudomyrmex triplarinus reduces the symptoms and swelling of rheumatoid arthritis. The cells that produce the venom were dissected from larval and pupal ants and culture conditions studied. Cell dissociation, with minimal amount of damage, was done with 0.25% trypsin at 4 degrees C with subsequent use of soybean trypsin inhibitor. A new medium was formulated and epidermal growth factor, fibroblast growth factor, insulin, cAMP, cGMP, and isoproterenol were beneficial. The optimum osmotic pressure was a relatively high 500 mOSM. Conditioning the medium with an established insect cell line was essential for long-term cell survival. Under these culture conditions the structural and metabolic integrity of the cells were maintained for up to 12 mo.
来自三脉伪切叶蚁的毒液可减轻类风湿性关节炎的症状和肿胀。从幼虫和蛹蚁中解剖出产生毒液的细胞，并对培养条件进行了研究。使用0.25%的胰蛋白酶在4摄氏度下进行细胞解离，损伤最小，随后使用大豆胰蛋白酶抑制剂。配制了一种新的培养基，表皮生长因子、成纤维细胞生长因子、胰岛素、环磷酸腺苷、环磷酸鸟苷和异丙肾上腺素都有帮助。最佳渗透压相对较高，为500毫渗摩尔。用已建立的昆虫细胞系对培养基进行预处理对于细胞的长期存活至关重要。在这些培养条件下，细胞的结构和代谢完整性可维持长达12个月。
