van der Zee R, Welling G W
J Chromatogr. 1985 May 29;325(1):187-94. doi: 10.1016/s0021-9673(00)96019-9.
Reversed-phase high-performance liquid chromatography with microbore columns (50 X 1.0 mm) was used effectively for the separation and analysis of proteins down to 1 ng at flow-rates of 0.1-0.2 ml/min. With the use of standard low-pressure gradient HPLC equipment, the peak volumes were five times smaller when compared with a conventional column at equal chromatographic efficiencies and analysis time. The sensitivity of detection was further increased by a reduction in solvent peaks, resulting in a 20-fold overall increase.
采用内径为50×1.0mm的微径柱进行反相高效液相色谱，能有效地分离和分析低至1ng的蛋白质，流速为0.1 - 0.2ml/min。使用标准的低压梯度高效液相色谱设备，在同等色谱效率和分析时间下，与传统色谱柱相比，峰体积小了五倍。通过减少溶剂峰进一步提高了检测灵敏度，总体提高了20倍。
