Moore R M, Walters R R
J Chromatogr. 1984 Dec 28;317:119-28. doi: 10.1016/s0021-9673(01)91652-8.
The resolution of cytochrome c, bovine serum albumin and ovalbumin on reversed-phase columns under gradient elution conditions was found to be constant or to improve as column length was decreased from 45 to 6.3 mm. The resolution remained constant even when the column length decreased to 1.6 mm. Recovery of protein in the first gradient cycle was improved by the use of short columns.
发现在梯度洗脱条件下，当反相柱长度从45毫米减小到6.3毫米时，细胞色素c、牛血清白蛋白和卵清蛋白的分离度保持不变或有所提高。即使柱长减小到1.6毫米，分离度仍保持不变。使用短柱可提高第一个梯度循环中蛋白质的回收率。
