School of Environment and Energy, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou, 510006, PR China; The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou, 510006, PR China.
School of Environment and Energy, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou, 510006, PR China; The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou, 510006, PR China.
Chemosphere. 2018 Oct;209:35-43. doi: 10.1016/j.chemosphere.2018.06.093. Epub 2018 Jun 14.
Polluted waters with a high residue of tetracycline also have a high concentration of nitrate. Thus, screening for both, highly efficient tetracycline biodegradation and nitrate transformation, is a key technical strategy. In this study, a novel tetracycline degrading strain, SQY5, which was identified as Klebsiella sp., was isolated from municipal sludge. Biodegradation characteristics of tetracycline were studied under various environmental conditions; including inoculation dose (v/v), initial tetracycline concentration, temperature, and pH. Response surface methodology (RSM) analysis demonstrated that the maximum degradation ratio of tetracycline can be obtained under the condition with an initial tetracycline concentration of 61.27 mg L, temperature of 34.96 °C, pH of 7.17, and inoculation dose of 29.89%. Furthermore, this was the first report on the relationship between the degradation of tetracycline and the denitrification effect, showing that a maximum tetracycline reduction rate of 0.113 mg L·h and denitrification rate of 4.64 mg L·h were observed within 32 h and 92 h of SQY5 inoculation, respectively. The data of this study has the potential for use in engineering processes designed for the simultaneous biological removal of nitrates while degrading antibiotics.
受高浓度四环素残留污染的水体也含有高浓度的硝酸盐。因此，筛选高效的四环素生物降解和硝酸盐转化是一项关键的技术策略。在这项研究中，从城市污泥中分离出一株新型的四环素降解菌株 SQY5，鉴定为克雷伯氏菌属。研究了在不同环境条件下，包括接种量（v/v）、初始四环素浓度、温度和 pH 值，四环素的生物降解特性。响应面法（RSM）分析表明，在初始四环素浓度为 61.27mg/L、温度为 34.96°C、pH 值为 7.17、接种量为 29.89%的条件下，可获得最大的四环素降解比。此外，这是首次报道四环素降解与脱氮效果之间的关系，表明在 SQY5 接种 32h 和 92h 内，四环素的最大还原率和脱氮率分别为 0.113mg/L·h 和 4.64mg/L·h。本研究的数据可用于设计同时去除硝酸盐和降解抗生素的生物工程过程。
