Hortofruticulture Department, Agrifood Research and Technology Centre of Aragon (CITA), Av. Montañana 930, 50059, Zaragoza, Spain.
Department of Fruit Quality and Biotechnology, Instituto de Biología Molecular y Celular de Plantas (UPV-CSIC), Ingeniero Fausto Elio, s/n 46022 Valencia, Spain.
J Plant Physiol. 2018 Sep;228:134-149. doi: 10.1016/j.jplph.2018.06.004. Epub 2018 Jun 9.
Analysis of the transcriptomic changes produced in response to hypoxia in root tissues from two rootstock Prunus genotypes differing in their sensitivity to waterlogging: resistant Myrobalan 'P.2175' (P. cerasifera Erhr.), and sensitive 'Felinem' hybrid [P. amygdalus Batsch × P. persica (L.) Batsch] revealed alterations in both metabolism and regulatory processes. Early hypoxia response in both genotypes is characterized by a molecular program aimed to adapt the cell metabolism to the new conditions. Upon hypoxia conditions, tolerant Myrobalan represses first secondary metabolism gene expression as a strategy to prevent the waste of resources/energy, and by the up-regulation of protein degradation genes probably leading to structural adaptations to long-term response to hypoxia. In response to the same conditions, sensitive 'Felinem' up-regulates a core of signal transduction and transcription factor genes. A combination of PLS-DA and qRT-PCR approaches revealed a set of transcription factors and signalling molecules as differentially regulated in the sensitive and tolerant genotypes including the peach orthologs for oxygen sensors. Apart from providing insights into the molecular processes underlying the differential response to waterlogging of two Prunus rootstocks, our approach reveals a set of candidate genes to be used expression biomarkers for biotech or breeding approaches to waterlogging tolerance.
分析根组织在缺氧条件下产生的转录组变化，这两种砧木基因型对水淹的敏感性不同：抗水淹的西洋李‘P.2175’（P. cerasifera Erhr.）和敏感的‘Felinem’杂种[P. amygdalus Batsch × P. persica (L.) Batsch]，揭示了代谢和调控过程的变化。两种基因型的早期缺氧反应都具有一个分子程序，旨在使细胞代谢适应新的条件。在缺氧条件下，耐受西洋李首先抑制次生代谢基因的表达，作为防止资源/能量浪费的策略，并通过上调蛋白降解基因，可能导致对长期缺氧反应的结构适应。对相同条件的反应，敏感的‘Felinem’上调了一组信号转导和转录因子基因。PLS-DA 和 qRT-PCR 方法的组合揭示了一组在敏感和耐受基因型中差异调控的转录因子和信号分子，包括桃的氧气传感器同源物。除了深入了解两种李砧木对水淹的不同反应的分子过程外，我们的方法还揭示了一组候选基因，可作为水淹耐受的生物技术或育种方法的表达生物标志物。
