Department of Biochemistry, University of Lucknow, Lucknow, Uttar Pradesh, India.
Biotechnology Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, Uttar Pradesh, India.
PLoS One. 2019 Feb 6;14(2):e0210903. doi: 10.1371/journal.pone.0210903. eCollection 2019.
Ocimum tenuiflorum is a widely used medicinal plant since ancient times and still continues to be irreplaceable due to its properties. The plant has been explored chemically and pharmacologically, however, the molecular studies have been started lately. In an attempt to get a comprehensive overview of the abiotic stress response in O. tenuiflorum, de novo transcriptome sequencing of plant leaves under the cold, drought, flood and salinity stresses was carried out. A comparative differential gene expression (DGE) study was carried out between the common transcripts in each stress with respect to the control. KEGG pathway analysis and gene ontology (GO) enrichment studies exhibited several modifications in metabolic pathways as the result of four abiotic stresses. Besides this, a comparative metabolite profiling of stress and control samples was performed. Among the cold, drought, flood and salinity stresses, the plant was most susceptible to the cold stress. Severe treatments of all these abiotic stresses also decreased eugenol which is the main secondary metabolite present in the O. tenuiflorum plant. This investigation presents a comprehensive analysis of the abiotic stress effects in O. tenuiflorum. Current study provides an insight to the status of pathway genes' expression that help synthesizing economically valuable phenylpropanoids and terpenoids related to the adaptation of the plant. This study identified several putative abiotic stress tolerant genes which can be utilized to either breed stress tolerant O. tenuiflorum through pyramiding or generating transgenic plants.
罗勒是一种从古至今被广泛应用的药用植物，由于其特性，至今仍无可替代。该植物已在化学和药理学方面进行了研究，但分子研究最近才开始。为了全面了解罗勒在非生物胁迫下的反应，我们对植物叶片在冷、干旱、洪水和盐胁迫下进行了从头转录组测序。我们对每个胁迫条件下与对照条件下的共同转录本进行了比较差异基因表达（DGE）研究。KEGG 通路分析和基因本体（GO）富集研究显示，由于四种非生物胁迫，代谢途径发生了几种修饰。除此之外，我们还对胁迫和对照样品进行了比较代谢组学分析。在冷、干旱、洪水和盐胁迫中，植物对冷胁迫最敏感。所有这些非生物胁迫的严重处理也降低了丁香酚，丁香酚是罗勒植物中主要的次生代谢物。本研究对罗勒的非生物胁迫影响进行了全面分析。目前的研究提供了对途径基因表达状态的深入了解，这些基因有助于合成与植物适应相关的经济价值较高的苯丙烷类和萜类化合物。本研究鉴定了几个可能的非生物胁迫耐受基因，这些基因可用于通过叠加或生成转基因植物来培育耐胁迫的罗勒。
